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EC number: 700-404-3 | CAS number: 127929-37-7
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: HET-CAM-TEST following OECD 405 Draft Guideline dated 24.4.2002
- Deviations:
- no
- Principles of method if other than guideline:
- The in vitro study was performed to assess the irritating potential of a test item by detection of damages in blood vessels under the chorionallantoic membran (CAM) of incubated chicken eggs (nine days old). The study is performed for notification according to Chemical Act.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2-bis({[3-(4-ethylpiperazin-1-yl)propanoyl]oxy}methyl)butyl 3-(4-ethylpiperazin-1-yl)propanoate
- EC Number:
- 700-404-3
- Cas Number:
- 127929-37-7
- Molecular formula:
- C33H62N6O6
- IUPAC Name:
- 2,2-bis({[3-(4-ethylpiperazin-1-yl)propanoyl]oxy}methyl)butyl 3-(4-ethylpiperazin-1-yl)propanoate
Constituent 1
Test animals / tissue source
- Species:
- other: Lohmann Leghorn chicken eggs
- Strain:
- other: Lohmann Leghorn
Test system
- Vehicle:
- physiological saline
- Controls:
- no
- Observation period (in vivo):
- 5 min.
Results and discussion
Any other information on results incl. tables
| Test group | Mean irritation score | Classification |
| Negativ Control | 00.00 | not irritant |
| Positive Control (0.1 n NaOH) | 19.70 | severely irritant |
| Positive Control (1 % SDS) | 10.23 | severely irritant |
| Test Item B 1061 | 10.29 | severely irritant |
The positive controls induced a severe irritation on the blood vessels. The negative control showed no irritation, score for the positive control lay within the demanded range.
The test item B 1061 was tested pure. A mean irritation score of 10.29 was calculated, corresponding to a classification as severely irritant.
Applicant's summary and conclusion
- Interpretation of results:
- highly irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- The test item B 1061 showed severe irritation in the HET-CAM-Test.
- Executive summary:
One valid experiment was performed.
Hen's eggs with an age of nine days were usedr.
The test item B 1061 was brought onto the surface of the CAM of a hen's egg which had been incubated at 37.5 +-0.5 °C for nine days.
Observation time was 5 minutes at room temperature.
Physiological sodium chloride solution was used as negative control. The negative control showed no irritating effect on the blood vessels under the membran.
Solutions of SDS (1 %) and NaOH (0.1 n) were used as positive controls. The positive controls induced a severe irritation on the blood vessels.
The test item B 1061 showed severe irritation on the blood vessels of the CAM. The calculated mean irritation score is 10.29.
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