Tris(2-hydroxyethyl)methylammonium methyl sulphate

Endpoint summary

Additional information

Short-term toxicity to fish:
The acute toxic effect of the test item to Zebra fish (Brachydanio rerio) over a 96 hour static exposure was assessed according to OECD guideline 203 and EU Method C.1. Zebra fish were exposed over a 96 hour period to the concentrations 50, 100, 5000 an 10000 mg/L and a control. Additionally the results were calculated also related to the analytically determined values. Based on the nominal concentrations the LC50 (96 h) is > 5000 < 10000 mg/L. Mortality (1/10) occurred from the concentration 5000 mg/L upward. Another substance-related effect was the symptom apathy. The NOEC (96 h) based on nominal concentrations is 100 mg/L. Based on the analytically determined concentrations the LC50 (96 h) is > 5000 < 10000 mg/L and the NOEC (96 h) is 103 mg/L.

Short-term toxicity to aquatic invertebrates:
The acute toxicity of the test substance to Daphnia sp. was determined in a 48-hour acute toxicity test according to DIN 38412 (daphnia-shorttime-test) and corresponding to the OECD Guideline No. 202. The test animals (10 per test concentration; 2 parallel assays, each) were exposed to different concentrations of the test substance in water. The percentage of animals unable to swim was determined in the individual test vessels after a 24 and 48 hours test duration. The test compound has a low acute toxicity towards daphnia with EC0 of 180 mg/L, EC50 of 350 mg/L and EC100 of 700 mg/L.

Toxicity to algae and cyanobacteria:
The influence of the test item on the growth of Scenedesmus subspicatus was investigated in a 96 hours toxicity test according to DIN 38412 and OECD Guideline No. 201. The test organismus, Scenedesmus subspicatus was cultivated for several generations in nutrient solution in the presence of different concentrations of the test substance. The chronic effect concentration of the test substance on algae was determined by measuring the turbidity of the algal suspensions after 96 hours. The following effect concentrations was determined:  EC0 = 300 mg/L EC10 = 380 mg/L EC 50 >1000 mg/L EC100 >1000 mg/L

Toxicity to microorganisms:
In an activated sludge respiration inhibition test the influence of the test item on the activated sludge by measuring the respiration rate was evaluated according to annex of EEC Directive 88/302/EEC, corresponding to OECD Guideline 209 and ISO Standard 8192. The respiration rate (oxygen consumption) of an aerobic activated sludge was measured in the presence of various concentrations of the test item after an incubation period of 30 minutes. The following test concentrations were used: 1, 10, 100 and 1000 mg test item/L. No significant inhibition of respiration was measured up to the highest tested concentration of 1000 mg/L (nominal). The EC20 in the activated sludge respiration inhibition test is >100 mg/L. 3,5 -Dichlorophenol was tested as a reference substance. The 30 min EC50 for the positive control 3,5-Dichlorophenol, was found to be 8 mg/L and is within the range of 5 – 30 mg/L recommended by the test guidelines; thus, confirming suitability of the activated sludge.