Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26.04.2016 - 06.05.2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

1
Chemical structure
Reference substance name:
1,3,5-Benzenetricarboxylic acid, copper(2+) salt (2:3)
Cas Number:
309721-49-1
Molecular formula:
C9 H6 O6 . 3/2 Cu
IUPAC Name:
1,3,5-Benzenetricarboxylic acid, copper(2+) salt (2:3)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Batch No.of test material: AP 199.6



Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
160 - 180 mg/L CaCO3
Test temperature:
18 - 22 °C +/- 1 °C
pH:
8.00 - 8.23
Dissolved oxygen:
7.51 - 8.30 [mg/L]
Nominal and measured concentrations:
Nominal loading levels [mg/L]: 1.00, 0.500, 0.250, 0.125, 0.0625, Control
Measured concentrations of Copper at the end of exposure [µg/L]: 190, 100, 42, 21, 11, 0.4 (control)

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
0.153 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion