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EC number: 943-265-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- The study integrity was not adversely affected by this deviation.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- yes
- Remarks:
- The study integrity was not adversely affected by this deviation.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 3706OB
- Expiration date of the lot/batch:31 March 2019
- Purity test date:> 99%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:At room temperature - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- -Source: The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Treatment: The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was determined to be 4.5 g/l in the concentrated sludge. Before use, the sludge was allowed to settle (42 minutes) and the supernatant liquid was used as inoculum at the amount of 10 ml/l of mineral medium. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
- Initial conc.:
- 17 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
The test item was a white powder with a purity of >99%. The test substance was tested in duplicate at 17 mg/l, corresponding to 10 mg TOC/l. The organic carbon content was based on the molecular formula taking into account the mole ratio of the components.
Since the test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components (test substance bottle A: 34.21 mg; test substance bottle B: 33.98 mg and toxicity control bottle: 33.89 mg). To this end, 10 ml of Milli-RO water was added to each weighing bottle containing the test substance. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test. Furthermore, the test medium was daily swirled around to ensure optimal contact between the test substance and test medium, since the test substance tended to float on the water surface.
Stock solutions of A) 8.50 g KH2PO4
mineral components 21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-RO water and made up to 1 litre,
pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-RO water
and made up to 1 litre.
C) 36.40 g CaCl2.2H2O dissolved in Milli-RO water and
made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-RO water and
made up to 1 litre.
Mineral medium 1 litre mineral medium contains: 10 ml of solution (A),
1 ml of solutions (B) to (D) and Milli-RO water.
Barium hydroxide 0.0125 M Ba(OH)2 (Boom, Meppel, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from the air.
Synthetic air (CO2 < 1 ppm) A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was sparged through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 ml/min).
Illumination The test media were excluded from light
TEST SYSTEM
- Pre-incubation medium:
The day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and inoculum (1% of final volume) were added to each bottle. This mixture was aerated with synthetic air overnight to purge the system of CO2.
- Type and number of bottles:
>Test suspension: containing test substance and inoculum (2 bottles).
>Inoculum blank: containing only inoculum (2 bottles)
>Positive control: containing reference substance and inoculum (1 bottle).
>Toxicity control: containing test substance, reference substance and inoculum (1 bottle).
-Preparation: At the start of the test (day 0), test and reference substance were added to the bottles containing the microbial organisms and mineral components.
The volumes of suspensions were made up to 2 litres with Milli-RO water, resulting in the mineral medium described before.
Three CO2-absorbers (bottles filled with 100 ml 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3
- Sampling time:
- 28 d
- Details on results:
- THEORETICAL CO2 PRODUCTION:
The ThCO2 of teh test item was calculated to be 2.12 mg CO2/mg.
The ThCO2 of sodium acetate was calculated to be 1.07 mg CO2/mg.
BIODEGRADATION:
The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation ofthe test item (1% and 3%, based on ThCO2).
In the toxicity control, more than 25% biodegradation occurred within 14 days (32%, based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity.
Functioning of the test system was checked by testing the reference substance sodium acetate, which showed a normal biodegradation curve
TEMPERATURE AND PH
The temperature recorded in a vessel with water in the same room varied between 21.6 and 22.5°C.
The pH values ranged from 7.6 at the start of the test to 7.5 on day 28. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item was not readily biodegradable under the conditions of the modified Sturm test presently performed.
- Executive summary:
The determination of the "ready" biodegradability of the test item was performed using the carbon dioxide (CO2) evolution test (modified Sturm test) according to OECD guideline No. 301 B, 1992. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-C, ISO 9439, 1999 and ISO 10634, 1995.
The test substance was tested in duplicate at 17 mg/l, corresponding to 10 mg TOC/l. The organic carbon content was based on the molecular formula taking into account the mole ratio of the components. The Theoretical CO2 production (ThCO2) of test item was calculated to be 2.12 mg CO2/mg.
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (Test item),
- 1 positive control (sodium acetate) and
- 1 toxicity control (Test item plus sodium acetate).
Since the test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts were added to the 2-litres test bottles containing medium with microbial organisms and mineral components. To this end, 10 ml of Milli-RO water was added to each weighing bottle containing the test substance. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test. Furthermore, the test medium was daily swirled around to ensure optimal contact between the test substance and test medium, since the test substance tended to float on the water surface. Test duration was 28 days (last CO2 measurement on day 29). The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test item (1% and 3%, based on ThCO2). In the toxicity control, the test item Reaction mass of AminoPhosphonium salt and Bisphenol AF (XA 31) was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid.
Reference
Table 1: CO2production and percentage biodegradation of the positive control substance.
Day |
HCl (0.05N) titrated (ml) |
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation * (%) |
|
|
Blank (mean)
|
Positive control |
|
|
|
|
2 |
44.98 |
39.33 |
5.65 |
6.2 |
6.2 |
7 |
5 |
43.74 |
22.71 |
21.03 |
23.1 |
29.3 |
34 |
7 |
43.44 |
30.77 |
12.67 |
13.9 |
43.3 |
51 |
9 |
43.69 |
37.33 |
6.36 |
7.0 |
50.3 |
59 |
14 |
42.67 |
37.29 |
5.38 |
5.9 |
56.2 |
66 |
* Calculated as the ratio between CO2produced (cumulative) and the ThCO2of sodium acetate: 85.5 mg CO2/2l
Table 2: CO2production and percentage biodegradation of the test substance (bottle A)
Day
|
HCl (0.05 N) titrated (ml) |
|
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation * (%) |
|
Blank (mean) |
Bottle A |
|
|
|
|
2 |
44.98 |
45.01 |
0.00 |
0.0 |
0.0 |
0 |
5 |
43.74 |
43.63 |
0.11 |
0.1 |
0.1 |
0 |
7 |
43.44 |
45.08 |
0.00 |
0.0 |
0.1 |
0 |
9 |
43.69 |
44.19 |
0.00 |
0.0 |
0.1 |
0 |
14 |
42.67 |
43.77 |
0.00 |
0.0 |
0.1 |
0 |
19 |
42.31 |
43.17 |
0.00 |
0.0 |
0.1 |
0 |
23 |
40.45 |
43.25 |
0.00 |
0.0 |
0.1 |
0 |
27 |
41.10 |
42.50 |
0.00 |
0.0 |
0.1 |
0 |
29 |
42.83 |
42.33 |
0.50 |
0.6 |
0.7 |
1 |
29 |
44.88 |
45.69 |
0.00 |
0.0 |
0.7 |
1 |
29 |
46.78 |
47.25 |
0.00 |
0.0 |
0.7 |
1 |
*Calculated as the ratio between CO2 produced (cumulative) and the ThCO2 of the test substance: 72.5 mg CO2/2l
Table 3: CO2production and percentage biodegradation of the test substance (bottle B).
Day
|
HCl (0.05 N) titrated (ml) |
|
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation* (%) |
|
Blank (mean) |
Bottle B |
|
|
|
|
2 |
44.98 |
44.15 |
0.83 |
0.9 |
0.9 |
1 |
5 |
43.74 |
43.11 |
0.63 |
0.7 |
1.6 |
2 |
7 |
43.44 |
43.28 |
0.16 |
0.2 |
1.8 |
2 |
9 |
43.69 |
44.80 |
0.00 |
0.0 |
1.8 |
2 |
14 |
42.67 |
43.10 |
0.00 |
0.0 |
1.8 |
2 |
19 |
42.31 |
43.23 |
0.00 |
0.0 |
1.8 |
2 |
23 |
40.45 |
42.30 |
0.00 |
0.0 |
1.8 |
2 |
27 |
41.10 |
42.85 |
0.00 |
0.0 |
1.8 |
2 |
29 |
42.83 |
42.96 |
0.00 |
0.0 |
1.8 |
2 |
29 |
44.88 |
45.83 |
0.00 |
0.0 |
1.8 |
2 |
29 |
46.78 |
46.24 |
0.54 |
0.6 |
2.4 |
3 |
*Calculated as the ratio between CO2 produced (cumulative) and the ThCO2 of the test substance: 72.0 mg CO2/2l
Table 4: CO2production and percentage biodegradation of the toxicity control.
Days |
HCl (0.05N) titrated (ml)
|
Produced CO2 (ml HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation*(%) |
|
|
Blank (mean) |
Toxicity control |
|
|
|
|
2 5 7 9 14 |
44.98 43.74 43.44 43.69 42.67 |
39.82 28.90 32.28 37.13 35.09 |
5.16 14.84 11.16 6.56 7.58 |
5.7 16.3 12.3 7.2 8.3 |
5.7 22.0 34.3 41.5 49.8 |
4 14 22 26 32 |
*Calculated as the ratio between CO2 produced (cumulative) and the sum of the ThCO2 of the test substance and positive control: 157.3 mg CO2/2l (ThCO2 test substance: 71.8 mg CO2/2l + ThCO2 sodium acetate: 85.5 mg CO2/2l)
Table 5 Comparison of biodegradation of the test substance in bottles A and B.
Day |
Biodegradation (%) |
|||
|
Bottle A |
Bottle B |
Mean A andB |
∆ A-B* |
2 |
0 |
1 |
1 |
1 |
5 |
0 |
2 |
1 |
2 |
7 |
0 |
2 |
1 |
2 |
9 |
0 |
2 |
1 |
2 |
14 |
0 |
2 |
1 |
2 |
19 |
0 |
2 |
1 |
2 |
23 |
0 |
2 |
1 |
2 |
27 |
0 |
2 |
1 |
2 |
29 |
1 |
2 |
2 |
1 |
29 |
1 |
2 |
2 |
1 |
29 |
1 |
3 |
2 |
2 |
*Absolute difference in biodegradation between bottles A and B
Description of key information
The Reaction mass of AminoPhosphonium salt and BisphenolAF (XA 31) was not readily biodegradable under the conditions of the carbon dioxide (CO2) evolution test (modified Sturm test) (OECD guideline No. 301 B)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
One reliable study is available for the Reaction mass of AminoPhosphonium salt and BisphenolAF (XA 31) for this endpoint. The determination of the "ready" biodegradability of the test item was performed using the carbon dioxide (CO2) evolution test (modified Sturm test) according to OECD guideline No. 301 B, 1992. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No. 440/2008 of 30 May 2008, Publication No. L142, Part C.4-C, ISO 9439, 1999 and ISO 10634, 1995.
The test substance was tested in duplicate at 17 mg/l, corresponding to 10 mg TOC/l. The organic carbon content was based on the molecular formula taking into account the mole ratio of the components. The Theoretical CO2 production (ThCO2) of test item was calculated to be 2.12 mg CO2/mg.
The study consisted of six bottles:
- 2 inoculum blanks (no test substance),
- 2 test bottles (Test item),
- 1 positive control (sodium acetate) and
- 1 toxicity control (Test item plus sodium acetate).
Test duration was 28 days (last CO2 measurement on day 29). The relative biodegradation values calculated from the measurements performed during the test period revealed no significant biodegradation of the test item (1% and 3%, based on ThCO2). In the toxicity control, the test item was found not to inhibit microbial activity.
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