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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vitro / ex vivo
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Justification for type of information:
Octafluoropropane is a gas, making testing difficult. However, numerous studies have shown that saturated perfluorocarbons as a class as not irritating to skin.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2011
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009
Reference Type:
study report
Title:
Unnamed
Year:
1995
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report Date:
1985
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report Date:
1984
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
EPISKIN(TM) reconstructed human epidermis was treated for a period of 15 minutes, followed by an incubation period of 42 hours. Cell viability was measured colorimetrically by the enzymatic reduction of yellow MTT tetrazolium salt.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: volatile

In vitro test system

Test system:
human skin model
Source species:
human
Cell type:
non-transformed keratinocytes
Vehicle:
unchanged (no vehicle)
Control samples:
yes, concurrent negative control
yes, concurrent positive control
Amount/concentration applied:
10 microlitre
Duration of treatment / exposure:
15 minutes
Duration of post-treatment incubation (if applicable):
42 hours
Number of replicates:
3

Results and discussion

In vitro

Results
Irritation / corrosion parameter:
% tissue viability
Run / experiment:
mean
Value:
ca. 91.7
Remarks on result:
no indication of irritation
Other effects / acceptance of results:
The relative mea viability of the test item treated tissues was 91.7% after a 15 minute exposure period. The quality criteria for acceptance of results in the test were satisfied.

Applicant's summary and conclusion

Conclusions:
The test item was considered to be non-irritant. Together with the results from other saturated perfluorocarbons, we can be confident octafluoropropane is not irritating.
Executive summary:

Octafluoropropane is non-irritant.