2-piperidin-2-ylethanol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-piperidin-2-ylethanol

EC Number:

216-059-2

EC Name:

2-piperidin-2-ylethanol

Cas Number:

1484-84-0

Molecular formula:

C7H15NO

IUPAC Name:

2-piperidin-2-ylethanol

Constituent 2

Reference substance name:

2-Piperidineethanol

IUPAC Name:

2-Piperidineethanol

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

Pseudokirchneriella subcapitata HINDÁK, SAG 61.81;

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

0.24 mmol Ca+Mg/L.

Test temperature:

Temperature (target) Nominal range: 21 - 24°C, controlled at ± 2°C

pH:

8.1 +- 0.2

Nominal and measured concentrations:

5 concentrations were tested in a geometrical series with a
dilution factor of 2: 6.25 - 12.5 - 25.0 - 50.0 - 100 mg/L.

Details on test conditions:

Biological Parameters
The cell density was measured daily via Chlorophyll-a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal.
Microscopic evaluation of the cells at the start and the end of the incubation period was carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algae cells.

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

The measured concentrations of the TS in the fresh media (0 h) were between 105 and 110 % of the nominal values. The measured concentrations in the old media (72 h) containing algae were in the range of 106 to 115 % of the nominal values.All effect values given are based on the nominal test item concentrations of 2-Piperidinethanol. For results, please refer toTable1.

	Growth Rate Inhibition Nominal test item concentration [mg/L]
NOEC	6.25
LOEC	12.5
ErC10	24.9 (21.4 – 28.5)
ErC20	37.3 (34.5 – 39.6)
ErC50	63.7 (60.3 – 67.8)
	Yield Inhibition Nominal test item concentration [mg/L]
NOEC	6.25
LOEC	12.5
EyC10	7.52 (< 6.25 – 11.6)
EyC20	13.5 (10.2 – 17.2)
EyC50	27.6 (23.9 – 31.7)