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Short-term toxicity to aquatic invertebrates

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Description of key information

At the limit concentration of the test item of 100 mg/L, no effect to Daphnia magna was observed. 

Key value for chemical safety assessment

Additional information

In the acute immobilization test with Daphnia magna (STRAUS) the effects test item at the limit test item concentration of 100 mg/L were determined according to OECD 202 (2004). The limit test was conducted under semi-static conditions over a period of 48 h.

Twenty daphnids were exposed to the limit concentration and the control. The Al3+content, component of the test item, was analytically verified with a cuvette test (cuvette test no. 1-02,Macherey-Nagel) by spectrophotometry in the fresh media (0 and 24 h) and the old media (24 and 48 h) of the limit concentration and the control.

The measured Al3+concentrations in the fresh media (0 and 24 h) and in the old media (24 and 48 h) were 102 % of the nominal value. All effect values given are based on the nominal concentration of the test item.

Water quality parameters (pH-value and dissolved oxygen concentration), measured in the fresh (0 and 24 h) and old media (24 and 48 h), were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.

EC-Values, NOEC and LOEC

(based on the nominal concentration of the limit test item concentration)

Test Duration

[h]

Nominal Concentration
of the Test Item

[mg/L]

Confidence Interval
p = 95%

[mg/L]

EC10/ EC50/ EC100

24

> 100

not applicable

48

> 100

not applicable

NOEC

48

  100

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LOEC

48

> 100

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At the limit concentration of the test item of 100 mg/L, no effects were observed on Daphnia magna.