Reaction mass of nonane-1,3-diyl diacetate and tetrahydro-3-pentyl-2H-pyran-4-yl acetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

EC Number:

242-640-5

EC Name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

Cas Number:

18871-14-2

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2,4-dideoxy-2-pentylpentitol

Constituent 2

Reference substance name:

Nonane-1,3-diyl diacetate

EC Number:

264-060-1

EC Name:

Nonane-1,3-diyl diacetate

Cas Number:

63270-14-4

Molecular formula:

C13H24O4

IUPAC Name:

nonane-1,3-diyl diacetate

impurity 1

Reference substance name:

1-(tetrahydrofuran-3-yl)hexyl acetate

Molecular formula:

C12H22O3

IUPAC Name:

1-(tetrahydrofuran-3-yl)hexyl acetate

impurity 2

Reference substance name:

rel-(3R,4R)-3-pentyltetrahydro-2H-pyran-4-ol

Cas Number:

24646-98-8

Molecular formula:

C10H20O2

IUPAC Name:

rel-(3R,4R)-3-pentyltetrahydro-2H-pyran-4-ol

impurity 3

Reference substance name:

3-hydroxynonyl acetate

Cas Number:

902270-36-4

Molecular formula:

C11H22O3

IUPAC Name:

3-hydroxynonyl acetate

Test material form:

liquid

Specific details on test material used for the study:

Not applicable

Method

Target gene:

TA1535, TA1537, TA98 TA100 and TA102

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

Not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9-mix (Aroclor-1254 induced rat liver S9-mix)

Test concentrations with justification for top dose:

Selection of an adequate range of doses was based on a dose range finding test with strain TA100 both with and without S9-mix. Eight concentraions were tested in triplicate. This dose range finding test was reported as a part of the direct plate assay and the preincubation assay respecitvely. The highest concentraion of JASMONYl used in the direct plate assay was the level at which the test substance exhbitited limited solubility and in the preincubation assay the level at which the test substance inhibited bacterial growth.

Vehicle / solvent:

Dimethyl Sulfoxide

Controls

Untreated negative controls:

yes

Remarks:

The vehicle of the test article, being dimethyl sulfoxide

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Remarks:

Saline (physicological saline) and DMSO were the positive controls used

Details on test system and experimental conditions:

At least five different doses (increasing with approximately half-log steps) of the test substance were tested in triplicate in each strain. The test substance was tested both in the absence and presence of S9-mix in each strain. The first experiment was a direct plate assay and the second experiment was a preincubation assay.

Evaluation criteria:

A Salmonella typhimurium reverse mutation assay is considered acceptable if it meets the follwing criteria:
a) The negative control data (number of spontaneous revertants per plate) should be within the laboratory background historical range for each tester strain.
b) The positive control chemicals should produce responses in all tester strains, which are within the laboratory historical range documented for each positive control substance.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 3

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 4

Species / strain:

S. typhimurium TA 102

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 5

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

JASMONYL did not induce a dose-related increase in the number of revertant (His+) colonites in each of the five tester strains (TA1535, TA1537, TA98, TA100 and TA102) both in the absence and presence of S9 -metabolic activation. These results were confirmed in independently repeated experiements.

Applicant's summary and conclusion

Executive summary:

Based on the results of the study it is concluded that JASMONYL is not mutagenic in the Salmonella typhimurium reverse mutation assay.