Registration Dossier
Registration Dossier
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EC number: 229-942-2 | CAS number: 6850-63-1
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 138.1 mg/L
Additional information
The assessment of the toxicity of 2,6-dimethylcyclohexylamine (CAS 6850-63-1) to aquatic microorganisms is based on experimental data from a test performed by BASF AG (1987; report no.: 9/0556/87) according to German Industrial Standard DIN 38412 part 8, using Pseudomonas putida as test organism. Based on growth inhibition a 17-h EC10 and 17-h EC50 were determined to be 138.1 mg/L and 188.3 mg/L, respectively.
In addition, there are supporting data available from a short-term respiration inhibition test conducted according to ISO 8192 (BASF AG, 1987; report no.: 01.269/87). In this limit test, the 30-min EC20 was determined to be >659 mg/L based on nominal test concentrations. As inoculum, industrial activated sludge was used.
According to the REACH guidance document (ECHA, 2016) chapter R.7b the results of a cell multiplication inhibition test with P. putida (Bringmann and Kühn, 1980; ISO 10712, 1996) should be used for calculation of the PNECmicro-organisms in cases where no other test results are available. However, as the short-term respiration inhibition test was performed with industrial activated sludge as inoculum, the results of the single-organism test with P. putida were selected as key data for the Chemical safety assessment (PNEC derivation).
In summary, it can be concluded that inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.
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