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Short-term toxicity to aquatic invertebrates

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Description of key information

Freshwater invertebrates
48 hour EC50 >100 mg/L (Daphnia magna), OECD 202, EU Method C.2, Sipos (2015)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Acute toxicity of the test material on Daphnia magna was assessed in an Acute immobilisation test, over an exposure period of 48 hours in a semi-static test system. The study was conducted under GLP conditions and in accordance to the standardised guidelines OECD 202 and EU Method C.2. The study was awarded a reliability score of 1 in accordance with the criteria for assessing data quality as set forth by Klimisch et al. (1997).

As no toxic response was observed during the preliminary range-finding test, only a limit test was carried out using only one test concentration (100 mg/L) and one control group in the definitive study in a semi-static system.

The test concentration was analytically determined at the beginning and at the end of the renewal periods. As the measured concentrations deviated not more than 20 per cent from the nominal, biological results are based on the nominal concentration.

Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentration and for the control.

All validity criteria were met during this study.

Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of Acid Blue 249 were the followings:

The 24h and 48h EC50 value: > 100 mg/L, the 48h EC100 value: > 100 mg/L, the 48h No-Observed Effect Concentration (NOEC): 100 mg/L (nominal), and the 48h Lowest Observed Effect Concentration (LOEC): > 100 mg/L (nominal).