Registration Dossier
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EC number: 274-402-1 | CAS number: 70210-05-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The biological biodegradation of FAT 21'020/C is 13.8 %.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Different available tests may be valuable, as part of a Weight of Evidence approach that gives information on biodegradability instead the Ready biodegradability test missing.
Inherent biodegradability
The inherent biodegradability was determined in a Zahn-Wellens, test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1992. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice (March 1986) issued by the Swiss Federal Department of the Interior recognizing the " OECD Principles of Good Laboratory Practice " Paris, 1981. The test substance and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC.
The biodegradation of the test substance after 28 days was 13.4 % while the biodegradation of the reference substance after 8 days was 99.5 %.
COD/BOD5 ratio
Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) are the most commonly used parameters for the characterization of the biodegradability capacity.
COD results are typically higher than BOD5 values, and the ratio between vary depending on the characteristics of the substrate. This ratio has been commonly used as an indicator for biodegradation capacity, the "Biodegradability index" (B.I.).
As indicated in ECHA Guidance requirement R.7B, the BOD5 test is a traditional biodegradation test that is now replaced by the ready biodegradability tests. Therefore, this test should not be performed today for assessment of the ready biodegradability of substances.
Older test data may, however, be used when no other degradability data are available.
Studies on BOD5 and COD are available for this substance, therefore they have been used together with the inherent biodegradability OECD 302.B in the weight of evidence approach for this substance.
COD was measured according to Method DEV DIN 38'409-H41-1 1980. Details of the method have been not reported. The COD measured was 1473 mg/g O2, at concentration of 2.5 mg/L.
BOD5 was measured according to ISO 5815. Details of the method have been not reported. The BOD5 measured was 11 mg/g O2, at concentration of 800 mg/L.
The ratio BOD5/COD is 0 and it indicates that the test substance Acid Violet 054 is not expected to be readily biodegradable
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