Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

During the evaluation of available data, Daphnia magna as well as Poecilia reticulate have been identified as most sensitive species. Based on the above acute LC50 values (below 1 mg/L) and considering the low biodegradability of the test substance, it needs to be classified and labelled as aquatic acute cat 1 (H400 Very toxic to aquatic life) and aquatic chronic cat. 1 (H410 Very toxic to aquatic life with long lasting effects) according to Regulation (EC) No 1272/2008 (CLP).

Additional information

There are several publications available describing the aquatic toxicity in daphnia and fish. Based on the reliability, the publication of Marchini et al. (1999) with daphnids has been identified as trigger for aquatic classification. In the fish publication (Könemann, H., 1981) including Poecilia reticulate (guppy) the determined 14-day EC50 value is 0.234 mg/L additionally supporting the trigger value of the daphnid study. The guppy value is below the LC50 value of the already available acute study of Bayer (96-h LC50 = 2.7 mg/L, zebra fish) showing a higher sensitivity of guppies compared to zebrafish. The 48 hour acute toxicity of alpha 2,4-Trichlorotoluene was further studied under static conditions equivalent to OECD guideline 202. Daphnids were exposed to a minimum of five concentrations (20 animals per concentration). Four replicates were performed for each concentration. The validity criteria were met as the immobilisation in the controls was < 10 % and the dissolved oxygen always maintained above 75 % saturation. A 48-hour LC50 of 0.12 to 0.13 mg/L was determined