Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: Isobutyl-tert.Butylether (IBTBE)
- purity: 86.4%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen .

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Details on test conditions:
- Test vessel : glass tubes sealed with gas unpermeable teflone caps (nominal volume 20 ml )
- Test volume : approx. 23 ml (brimful)
- Loading (animals/ml) : approx. 0,22
- Number of animals/vessel : 5
- Total number of animals/conc. : 20
- Number of replicates/conc . : 4
- Number of replicates/control : 4
- Number of replicates for the measurement of pH and 02 : 1
- Number of parallels without animals: 1(parallel 5) per test concentration and control for measurement of the pH and oxygen content at the beginning of the exposition. This parallel was thrown away after measurement. 4 per test concentration and control for concentration control analysis .
- Age of the animals at the begin of exposition : 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals : 2 - 4 weeks
- Illumination : Artificial light, type warm white (e .g. OSRAM L58 W31)
- day:night rhythm = 16 : 8 h
- Light intensity: .About 1- 8 NE/(mZ•s) at a wave length of 400 - 750 nm
- Test parameter : Swimming ability of the test animals
- Check of the study and recording (swimming ability) : Visually after 0, 24 and 48 h
- Feeding : None
- Measurement of temperature : Continuously during the whole test period in a separate vessel close the test vessels .
- Measurement of pH and oxygen : After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration .
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.11 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
1.75 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables


EC0 (48h) = 25 mg/l nominal (*0.07=1.75 mg/l effective)
EC50 (48h) = 58.7 mg/l nominal (*0.07=4.11 mg/l effective)
EC100 (48h) = 100 mg/l nominal (*0.07=7.0 mg/l effective)
24 h(nominal): EC0=25mg/l, EC50=60.4mg/l, EC100>100mg/l;

Applicant's summary and conclusion

Validity criteria fulfilled:
yes