Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study, validity criteria fulfilled;

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
Principles of method if other than guideline:
ISO 14593 - CO2-Headspace Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: Isobutyl-tert-Butylether,
- purity: 86.4%
- physical form: liquid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- inoculum: Activated sludge from a laboratory wastewater plant treating municipal sewage .
- Concentration of dry substance: 4 mg/I
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
27.4 mg/L
Based on:
test mat.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
< 10
Sampling time:
28 d
Details on results:
Kinetic of test substance (in %):
ca. 5 after 7 day(s)
ca. 5 after 14 day(s)
ca. 4 after 21 day(s)
ca. 3 after 28 day(s)
Kinetic of control substance (in %):
ca. 80 ... 90 after 14 day(s)

Any other information on results incl. tables

Test substance: volatile, no DOC values were measured;

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed