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Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
fungi
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from abstract of peer reviewed journal.
Qualifier:
according to
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Fungal growth inhibition determination of 2-methyl-9,10-anthraquinone by microplate plate.
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on sampling:
No data
Vehicle:
yes
Remarks:
dimethyl sulfoxide
Details on test solutions:
Title compound was dissolved in dimethyl sulfoxide
Test organisms (species):
Aspergillus niger
Details on inoculum:
Strain: N402
Test type:
static
Water media type:
freshwater
Total exposure duration:
40 h
Remarks on exposure duration:
No data
Post exposure observation period:
No data
Hardness:
No data
Test temperature:
37 deg C
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
Fungi incubated with title comp. at 37 deg C for 40 h.
Reference substance (positive control):
yes
Remarks:
H2O2 (Hydrogen peroxide)
Key result
Duration:
40 h
Dose descriptor:
other: Minimal inhibitory concentration (MIC)
Effect conc.:
25 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
No data
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
No data
Validity criteria fulfilled:
not specified
Conclusions:
The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l by microplate method after 40 hr of incubation.
Executive summary:

Fungal growth inhibition determination of test material was carried out by microplate plate. The test substance was dissolved in dimethyl sulphoxide. The test organism used was fungal species i.e. Aspergillus niger N402. The fungal species was incubated with title compound at 37 deg C for 40 h and growth inhibition was determined by measuring at 590 nm. The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l.

 

Description of key information

Toxicity to microorganisms:

Effect of test material was evaluated on microorganism based on the data from peer reviewed journal ,fungal growth inhibition determination of test material was carried out by microplate plate. The test substance was dissolved in dimethyl sulphoxide. The test organism used was fungal species i.e. Aspergillus niger N402. The fungal species was incubated with title compound at 37 deg C for 40 h and growth inhibition was determined by measuring at 590 nm. The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l.

In another data , the Minimal inhibitory concentration (MIC) value of the test substance to the fungal species Candida dubliniensis ATCC 777 was determined to be > 100 mg/ml.

 

Key value for chemical safety assessment

EC50 for microorganisms:
25 mg/L

Additional information

Toxicity to microorganisms:

Effect of test material was evaluated on microorganism based on the data from peer reviewed journal ,fungal growth inhibition determination of test material was carried out by microplate plate. The test substance was dissolved in dimethyl sulphoxide. The test organism used was fungal species i.e. Aspergillus niger N402. The fungal species was incubated with title compound at 37 deg C for 40 h and growth inhibition was determined by measuring at 590 nm. The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l.

In another data , the Minimal inhibitory concentration (MIC) value of the test substance to the fungal species Candida dubliniensis ATCC 777 was determined to be > 100 mg/ml.