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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Short term toxicity of test chemical to aquatic invertebrates was performed according to the OECD guideline 202 in a static system.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
yes
Remarks:
DMSO
Details on test solutions:
The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feedingACCLIMATION
- No data available
- Acclimation period:- Acclimation conditions (same as test or not)
- Type and amount of food:- Feeding frequency
-Health during acclimation (any mortality observed):
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
± 1 hr
Test temperature:
20±1°C
pH:
Test at higher concentration 0.80 mg/l: 7.9 changes to 7.8 during testControl: 7.8 changed to 7.9 Control + DMSO: 7.9 changes to 7.8
Dissolved oxygen:
higher than 7.6 mg/L at the end of test
Nominal and measured concentrations:
0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass vesse
l- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.As an example, the preparation of dilution water meeting the requirements is described below.Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.Prepare the solutions specified below:- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).MixingMix 2.5 ml of each of the four solutions and make up to 1 l with water.The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.- Hydrochloric acid, e.g. [HCl] : 1 mol/l.Reference substance: Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).OTHER TEST CONDITIONS- Adjustment of pH: no adjustment done- Photoperiod: No - Darkness- Light intensity:CALCULATION:EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.14 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CI was 0.12-0.17 mg/l
Results with reference substance (positive control):
- Results with reference substance valid- EC50: 0.76 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Executive summary:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in ay 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP criteria.

Description of key information

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in ay 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.14 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in ay 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP criteria.