Bis[3-[[4-[benzylmethylamino]phenyl]azo]-1,4-dimethyl-1H-1,2,4-triazolium] tetrachlorozincate(2-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From February 16, 1994 to February 18, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted on a similar substance, the complete justification for the Read Across approach is reported at section 13. The original Reliability of the test is 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Ciba Geigy AG testing facility of the Product Ecology TF, Textile Dyes Division
- Method of breeding: Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 I of reconstituted water
- Feeding during test:
- Food type: feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantités that the food was consumed within 24h.
- Frequency: daily

ACCLIMATION
- Acclimation period: 24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 710 µm sieve.
- Acclimation conditions: 18 - 22 °C, The water was renewed partially three times weekly.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Theoretical water hardness: 136 mg/l CaC03

Test temperature:

21 °C

pH:

7.7 - 8.2

Dissolved oxygen:

92-99%

Nominal and measured concentrations:

NOMINAL: 0.58, 1.0, 1.8, 3.2, 5.8 and 10.0 mg test article / I.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 ml bekers
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 - 200 ml test medium
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 0.5 g test substance were mixed with 200 ml test medium and fill up to 500 ml with test medium.
The stock solution was homogenized for 5 minutes in the ultrasonic bath.
The test medium used had the following composition :
NaHC03: 65 mg/l
MgS04 X 7 H20 123.3 mg/l
KCl 5.8 mg/l
CaCI2 X 2 H20 294 mg/l
Na2SiO3 X 9H20 10 mg/l
NaN03 274 µg/l
KH2P04 143 µg/l
K2HP04 184 µg/l
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4 :1
- Na / K ratio: 10:1
- Conductivity:
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no ,7.8 - 8.2.
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: no data

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0mg test article / I nominal.
- Results used to determine the conditions for the definitive study: yes, based on a pretest.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limit: 1.3549 - 1.6868

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

ca. 3.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

The substance shows a EC50(48h) = 1.5 mg/l

Executive summary:

The acute toxicity of test substance to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0 mg test article / l, and a control without any additions. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 80.0 - 120.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control non immobilization of the Daphnia magna was observed after 48 hours. The EC0 value is at least 1.0 mg/l, but might even be higher than this concentration. The EC100 value is 3.3 mg/l after 48 hours.

Results :

(24h) EC0: > 1.0 mg/l, EC50: 2.5 mg/l, EC100: 5.8 mg/l

(48h) EC0: > 1.0 mg/l, EC50: 1.5 mg/l, EC100: = 3.3 mg/l, NOEC= 1.0 mg/l

Description of key information

EC50= 1.5 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.5 mg/L

Additional information

Experiment was conducted on Similar Substance 2 according to the guideline OECD 202