2,2,2-trichloroethane-1,1-diol

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from database of Japan Ministry of the Environment

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: 2,2,2-Trichloroethane-1,1-diol (Chloral hydrate)
- Molecular formula: C2-H3-Cl3-O2
- Molecular weight: 165.4026g/mol
- Smiles notation: C(C(O)O)(Cl)(Cl)Cl
- InChl: RNFNDJAIBTYOQL-UHFFFAOYSA-N
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Food type: Unicellular green algae(Pseudokirchneriella subcapitata)
- Amount: At a rate of(Daphnia magna) one animal per 0.2 mg Carbon (organic carbon content) / day

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Hardness:

No data

Test temperature:

20±1 deg.C

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

6.25,12.5,25.0,50.0, l00 mg / L (common ratio: 2.0) and the control group

Details on test conditions:

TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate):to exchange a total volume of every 48 or 72 hour)
- No. of organisms per vessel: 1 Daphnia magna/container
- No. of vessels per concentration (replicates):10 container /test group
OTHER TEST CONDITIONS
- Photoperiod: Room light, 16-h light / 8 hours dark (bright; AM, 6: 00 ~ PM1O: 00),
- Light intensity: Photon flux density of test near the container: 9-15 µ mol • m-2 • s-1
TEST CONCENTRATIONS
- Test concentrations: 6.25,12.5,25.0,50.0, l00 mg / L (common ratio: 2.0) and the control group

Key result

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 96.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

65 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Breeding inhibition

Remarks on result:

other: 53.8‐81.9mg/L

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

11.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: survival and litter size

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

23.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: cumulative survival production litter size

Tests conducted before FY 2002 needs confirmation of test results, because some of these tests were conducted using a dispersant.

Validity criteria fulfilled:

not specified

Conclusions:

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Executive summary:

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Considering the NOEC value it is concluded that the test substance Cloral hydrate does not considered for long term aquatic toxicity.

Description of key information

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

11.5 mg/L

Additional information

In a Long-term toxicity to aquatic invertebrates study for Chloral hydrate in Daphnia magna when they were exposed for 21 days under semi -static condition, the Median lethal concentration LC50 was found to be >96.2mg/l at inhibition in breeding effect, whereas effect concentration (EC50) were found to be 65 mg/l based on inhibition in breeding (at 95% confidence limit is 53.8 to 81.9 mg/l) while low effect concentration (LOEC) on the cumulative survival production litter size was found to be 23.6 mg/l. And NOEC was found to be 11.5 mg/l based on survival and litter size.

Considering the NOEC value it is concluded that the test substance Cloral hydrate does not considered for long term aquatic toxicity.