Registration Dossier

Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
EFFECTS OF SELECTED HEAVY METALS ON EARLY LIFE OF SOCKEYE AND PINK SALMON
Author:
J. A. SERVIZI and D. W. MARTENS
Year:
1978
Bibliographic source:
Rep.No.39, Int.Pacific Salmon Fish.Comm.(Br.Col.): 26 p.

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned based
Principles of method if other than guideline:
Long term toxicity test was performed on Oncorhynchus gorbuscha for 7 days when exposed to Sulfuric acid, Mercury (2+) salt (7783-35-9).
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Mercury sulphate
EC Number:
231-992-5
EC Name:
Mercury sulphate
Cas Number:
7783-35-9
Molecular formula:
H2O4S.Hg
IUPAC Name:
mercury sulfate
Details on test material:
- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data availableNo data available

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No data available
- Eluate: No data available
- Differential loading: No data available
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Hydrochloric acid
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 5-10 ML/18 L STOCK
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Residue appeared

Test solutions

Vehicle:
not specified
Details on test solutions:
Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No data available
- Eluate: No data available
- Differential loading: No data available
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Hydrochloric acid
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 5-10 ML/18 L STOCK
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Residue appeared

Test organisms

Test organisms (species):
Oncorhynchus gorbuscha
Details on test organisms:
Details on test organisms
TEST ORGANISM
- Common name: Pink salmon
- Strain: No data available
- Source: natural spawning areas
- Age at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Weight at study initiation (mean and range, SD): No data available
- Method of breeding: No data available
- Feeding during test: No data available
- Food type: No data available
- Amount: No data available
- Frequency: 1

ACCLIMATION
- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food: No data available
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available
QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
7 d
Post exposure observation period:
No data available

Test conditions

Hardness:
83.1 mg/L CaCO3
Test temperature:
5.5- 8.8°C
pH:
7.63
Dissolved oxygen:
No data available
Salinity:
No data available
Nominal and measured concentrations:
No data available
Details on test conditions:
Details on test conditions
TEST SYSTEM
- Test vessel: incubators
- Type (delete if not applicable): open / closed: No data available
- Fill volume: 1.35 L
- Aeration: Yes
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter
- Renewal rate of test solution (frequency/flow rate): Water flow to each incubator was initially set at 32 ml/min but was increased to 65 ml/min to maintain dissolved oxygen near saturation throughout
the study
- No. of organisms per vessel: Three hundred sockeye salmon eggs
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): No data available
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: 62.5 mg/L CaCO3
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: 7.63 (7.55- 7.80)
- Photoperiod: No data available
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study No data available
- Test concentrations: 140ug/l
- Results used to determine the conditions for the definitive study: No data available
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
0.14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Details on result
- Mortality: 88%
- Overall mortality/survival: No data available
- Days to hatch and numbers hatched: No data available
- Data for length and weight of surviving fish: No data available
- Type of and number with morphological abnormalities: As growth proceeded, petechiae disappeared and malformed alevins occurred
- Type of and number with behavioural abnormalities: Teratogenic abnormalities
- Other biological observations: Mortality and malformation data indicate these toxic effects were greatest when exposure to mercury commenced soon after fertilization instead of after eyeing.
- Effect concentrations exceeding solubility of substance in test medium: No data available
- Incidents in the course of the test which might have influenced the results: No data available
Results with reference substance (positive control):
No data available
Reported statistics and error estimates:
No data available

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC 50 value for the Oncorhynchus gorbuscha for 7 days was found to be 0.14 mg/L (140 ug/L) when exposed to Sulfuric acid, Mercury (2+) salt (7783-35-9).
Executive summary:

Long term toxicity test was performed on Oncorhynchus gorbuscha for 7 days when exposed to Sulfuric acid, Mercury (2+) salt (7783-35-9). Hydrochloric acid was the chemical solvent. Fresh water was the media and mortality was the effect measured.

On the basis of mortality effect LC 50 value for the Oncorhynchus gorbuscha for 7 days was found to be 0.14 mg/L (140 ug/L) when exposed to Sulfuric acid, Mercury (2+) salt (7783-35-9).