Registration Dossier
Registration Dossier
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EC number: 203-254-2 | CAS number: 104-94-9
Long-term toxicity to fish
Administrative data
- Endpoint:
- long-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- p-anisidine
- EC Number:
- 203-254-2
- EC Name:
- p-anisidine
- Cas Number:
- 104-94-9
- Molecular formula:
- C7H9NO
- IUPAC Name:
- 4-methoxyaniline
- Details on test material:
- - Name of test material (as cited in study report): p-Anisidine
- Molecular formula: C7-H9-N-O
- Molecular weight: 123.1541g/mol
- Smiles notation: c1(ccc(cc1)N)OC
- InChl: 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- - Common name: Japanese killifish
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 24±1 degreeC
- Nominal and measured concentrations:
- 100, 50, 25.0, 12.5 mg/L (geometric ratio 2.0), control
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: test liquid volume 3.6 L per concentration (1.8 L per vessel)
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): continuous supply system of test solution
- No. of organisms per vessel: 20 per concentration (10 per vessel)
- No. of vessels per concentration (replicates): 2
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 70.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % CL 50 - 100 mg/L
Applicant's summary and conclusion
- Conclusions:
- LC50 values of p-Anisidine in Oryzias latipes were found to be >100mg/l for 14 days and 70.7mg/l for 21 days based on effect on mortality while
NOEC was found to be 12.5mg/l for 21 days exposure. - Executive summary:
Toxicity study of p-Anisidine in Oryzias latipes was conducted under flow –through condition for an exposure period of 14 and 21 days at dose concentrations of 0, 12.5, 25, 50 and 100 mg/L.
LC50 values of p-Anisidine in Oryzias latipes were found to be >100mg/l for 14 days and 70.7mg/l for 21 days based on effect on mortality while NOEC was found to be 12.5mg/l for 21 days exposure.
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