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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29.03.2017 - 31.03.2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Data is from ABITEC lab report.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Short term toxicity of 4-amino-m-toluenesulfonic acid to aquatic invertebrates was performed according to the OECD guideline in a static system.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: 4-amino-m-toluenesulfonic acid
- Molecular formula: C7-H9-N-O3-S
- Molecular weight: 187.218 g/mol
- SMILES : O=S(=O)(O)c(ccc(N)c1C)c1
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not required
Vehicle:
not specified
Details on test solutions:
The stock solution 150 mg/l was prepared by dissolving white powder in reconstituted water. Solution kept in ultrasonic bath for 20 min. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Remarks on exposure duration:
± 1 hr
Test temperature:
20 ± 1°C
pH:
Test at 150 mg/l: 4.8 (changed to 6.5 during test)
Control: 7.8 (Change to 7.6 during test)
Dissolved oxygen:
higher than 8 mg/L at the end of test
Nominal and measured concentrations:
30, 45, 67, 100, 150 mg/l nominal concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
74.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % CI was 62.9 - 89.2 mg/l
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance 4-amino-m-toluenesulfonic acid, in Daphnia magna was determined to be 74.9 mg/L on the basis of mobiity inhibition effects in a 48 hour study.
Executive summary:

Aim of the study was to determe the inhibition of the mobility of daphnids was carried out with the substance 4-amino-m-toluenesulfonic acid. Test was performed according to OECD Guideline 202.

The stock solution 150 mg/l was prepared by dissolving white powder in reconstituted water. Solution kept in ultrasonic bath for 20 min. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water and was tested at the nominal concentrations 30, 45, 67, 100, 150 mg/l . Effects on immobilisation were observed for 48 hours.

The median effective concentration (EC50) for the test substance 4-amino-m-toluenesulfonic acid, in Daphnia magna was determined to be 74.9 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.

Description of key information

Aim of the study was to determe the inhibition of the mobility of daphnids was carried out with the substance 4-amino-m-toluenesulfonic acid. Test was performed according to OECD Guideline 202. The stock solution 150 mg/l was prepared by dissolving white powder in reconstituted water. Solution kept in ultrasonic bath for 20 min. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water and was tested at the nominal concentrations 30, 45, 67, 100, 150 mg/l . Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance 4-amino-m-toluenesulfonic acid, in Daphnia magna was determined to be 74.9 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
74.9 mg/L

Additional information

Aim of the study was to determe the inhibition of the mobility of daphnids was carried out with the substance 4-amino-m-toluenesulfonic acid. Test was performed according to OECD Guideline 202.

The stock solution 150 mg/l was prepared by dissolving white powder in reconstituted water. Solution kept in ultrasonic bath for 20 min. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water and was tested at the nominal concentrations 30, 45, 67, 100, 150 mg/l . Effects on immobilisation were observed for 48 hours.

The median effective concentration (EC50) for the test substance 4-amino-m-toluenesulfonic acid, in Daphnia magna was determined to be 74.9 mg/L on the basis of mobiity inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.