1-dodecylpyridinium chloride

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Effect of Laurylpyridinium chloride was determined in larvae of M.Mercenaria (Clam) by continuous exposure of chemical.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other aquatic crustacea: M.Mercenaria (Clam)

Details on test organisms:

TEST ORGANISM
- Common name: quahog
- Age at study initiation (mean and range, SD): larvae
- Food type: a mixture of a flagellated algal food
- Amount: 0.01 ml of packed cell volume per 1-1 culture per day.
- Frequency: Daily

Test type:

semi-static

Water media type:

saltwater

Total exposure duration:

12 d

Remarks on exposure duration:

10 days of continuous exposure

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

0.0025, 0.005, 0.01, 0.025, 0.05, 0.10, 0.25, 0.50, 1.00, 2.50, 5.00, 10.00 mg/l

Details on test conditions:

Details on test conditions
TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): Renewed in every second days
- Test concentrations: 0.0025, 0.005, 0.01, 0.025, 0.05, 0.10, 0.25, 0.50, 1.00, 2.50, 5.00, 10.00 mg/l

Reference substance (positive control):

not specified

Key result

Duration:

12 d

Dose descriptor:

LOEC

Effect conc.:

0.05 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Key result

Duration:

12 d

Dose descriptor:

LOEC

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Survival

Validity criteria fulfilled:

not specified

Conclusions:

In long term toxicity to aquatic invertebrates study Low observed effect concentration (LOEC) of test chemical on M.Mercenaria (Clam) larvae was observed to be 0.25 mg/l and 0.050 mg/l based on survival and growth effects respectively for exposure period of 12 days.

Executive summary:

Long term toxicity test was performed on M.Mercenaria (Clam) larvae.10,000 to 15,000 larvae reared to the straight hinge larval stage. The cultures were fed a mixture of flagellated algal food and continued exposed to the test chemical for 12days. Effects on cultures based on survival and growth was determined.

Based on the results it was concluded that the endpoint for long term toxicity to aquatic invertebrates to test material was found to be LOEC at 0.25 mg/l and 0.050 mg/l concentrations based on survival and growth of M.Mercenaria (Clam) larvae.This LOEC value suggest that the test chemical was toxic to aquatic invertebrates in chronic exposure.

Description of key information

Long term toxicity test was performed on M.Mercenaria (Clam) larvae.10,000 to 15,000 larvae reared to the straight hinge larval stage. The cultures were fed a mixture of flagellated algal food and continued exposed to the test chemical for 12days. Effects on cultures based on survival and growth was determined.

Based on the results it was concluded that the endpoint for long term toxicity to aquatic invertebrates to test material was found to be LOEC at 0.25 mg/l and 0.050 mg/l concentrations based on survival and growth of M.Mercenaria (Clam) larvae.This LOEC value suggest that the test chemical was toxic to aquatic invertebrates in chronic exposure.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.25 mg/L

Additional information

In a peer reviewed journal study for 21 days was described for two aquatic invertebrates, M.Mercenaria (Clam) and C. Virginica(Oysters) larvae

Long term toxicity test was performed on M.Mercenaria (Clam) larvae.10,000 to 15,000 larvae reared to the straight hinge larval stage. The cultures were fed a mixture of flagellated algal food and continued exposed to the test chemical for 12days. Effects on cultures based on survival and growth was determined.

Based on the results it was concluded that the endpoint for long term toxicity to aquatic invertebrates to test material was found to be LOEC at 0.25 mg/l and 0.050 mg/l concentrations based on survival and growth of M.Mercenaria (Clam) larvae.This LOEC value suggest that the test chemical was toxic to aquatic invertebrates in chronic exposure.

Long term toxicity test was performed on C. Virginica(Oysters) larvae.10,000 to 15,000 larvae reared to the straight hinge larval stage. The cultures were fed a mixture of flagellated algal food and continued exposed to the test chemical for 14 days. Effects on cultures based on survival and growth was determined.

Based on the results it was concluded that the endpoint for long term toxicity to aquatic invertebrates to test material was found to be LOEC at 0.25 mg/l and 0.050 mg/l concentrations based on survival and growth of C. Virginica (Oysters) larvae.This LOEC value suggest that the test chemical was toxic to aquatic invertebrates in chronic exposure. Based on the above effect concentration we can consider the test material to be classiffied as aquatic chronic 3