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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Analytical monitoring:
not required
Remarks:
Test substance is stable in recommended storage conditions
Vehicle:
not specified
Details on test solutions:
The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.76 cm
- Weight at study initiation (mean and range, SD): average 0.473 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
152.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.7
Dissolved oxygen:
7.6 mg/l
Nominal and measured concentrations:
3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: LC50 was observed to be to be >12.5 mg/L but <25 mg/L.

8.1. Mortality and symptoms

Nominal

Number of

Mortality (cumulated) after

 

 

 

 

 

 

 

Concentration fish

3 h

 

 

6 h

 

 

 

24h

 

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

 

-

 

-

-

 

 

-

-

 

-

-

-

-

-

-

3.125

4

4

-

 

-

 

-

-

 

 

-

-

 

-

-

-

-

-

-

6.25

4

4

-

 

-

 

-

-

 

 

-

-

 

-

-

-

-

-

-

12.5

4

4

-

 

-

 

-

-

 

 

-

-

 

-

-

-

-

-

-

25

4

4

-

 

-

 

-

-

 

 

4

4

 

4

4

4

4

4

4

50

4

4

-

 

-

 

4

4

 

 

4

4

 

4

4

4

4

4

4

Visible Symptoms:

Nominal

Number of

 

 

 

 

 

 

 

 

3 h

 

6 h

 

 

24h

 

48h

72h

96h

 

Concentration

 

fish

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

 

(mg/l)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Control

4

4

NS4 NS4 NS4 NS4 NS4 NS4

NS4 NS4 NS4 NS4 NS4 NS4

 

3.125

4

4

NS4 NS4 NS4 NS4 NS4 NS4

NS4 NS4 NS4 NS4 NS4 NS4

 

6.25

4

4

NS4 NS4 M4 M4

 

M4

 

M4

 

NS4 NS4 NS4 NS4 NS4 NS4

 

12.5

4

4

NS4 NS4 M4

M4

 

M4

 

M4

 

M4

M4

M4

M4

M4

M4

 

25

4

4

M4

M4

 

IS4

IS4

 

-

 

-

 

-

-

-

-

-

-

 

50

4

4

IS4

IS4

 

-

-

 

-

 

-

 

-

-

-

-

-

-

 

Explanation of symptoms:

A: apathy

 N: Narcotic-like state

 C: Swimming near water surface

 M: Slow movements compared to control NS: No abnormal symptoms

S: Surfacing behaviour

 L: Loss of equilibrium SM: Shimming movement H1: Hyperactivity

 H2: Hypo activity

 B: Bending behavior

 F: Fin rot

 IS: Invert Swimming

 SB: Swimming behaviour

 RF: Respiratory Function

 P: Pigmentation

8.2. pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.68

7.69

8.07

8.08

8.22

8.23

8.29

8.28

3.125

4

4

7.73

7.75

8.09

8.10

8.21

8.22

8.28

8.30

6.25

4

4

7.79

7.81

8.15

8.16

8.23

8.24

8.31

8.34

12.5

4

4

7.85

7.89

8.20

8.21

8.25

8.27

8.36

8.38

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

 

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.1

7.1

6.3

6.3

6.0

6.0

6.0

6.0

3.125

4

4

6.8

6.8

6.4

6.4

5.8

5.8

5.5

5.5

6.25

4

4

6.8

6.8

6.3

6.2

5.8

5.8

5.5

5.5

12.5

4

4

6.8

6.8

6.4

6.4

5.9

5.9

5.4

5.4

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

8.3. Test temperature

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.5

23.5

22.0

22.0

22.3

22.3

21.6

21.6

3.125

4

4

23.7

23.7

22.3

22.3

22.5

22.5

22.9

22.9

6.25

4

4

23.6

23.6

22.3

22.3

22.5

22.5

22.9

22.9

12.5

4

4

23.6

23.6

22.3

22.3

22.5

22.5

22.9

22.9

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. on Zebra Fish Danio rerio was determine to be >12.5 mg/L but <25 mg/L.
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively . This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >12.5 mg/L but <25 mg/L. Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively .This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be>12.5 mg/L but <25 mg/L.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
12.5 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring. After that analytically detect that stock and set up test concentrations of 3.125mg/L,6.25mg/L,12.5mg/L,25mg/L,50mg/L ,respectively .This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be>12.5 mg/L but <25 mg/L.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.