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Short-term toxicity to aquatic invertebrates

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Description of key information

Exposure of Daphnia magna to the test item gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF OECD 202 and EU Method C.2).

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Additional information

GUIDELINE

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202 "Daphnia sp., Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No 440/2008.

METHODS

Due to the low solubility and complex nature of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF).

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to a Water Accommodated Fraction (WAF) of the test item at a nominal loading rate of 100 mg/L for 48 hours at a temperature of approximately 21°C under semi-static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.

RESULTS

Chemical analysis of the freshly prepared 100 mg/L loading rate WAF test preparations at 0 and 24 hours (fresh) showed measured test concentrations of 0.058 to1.0 and 0.060 mg/L respectively were obtained. A decline in measured test concentration was observed at 24 and 48 hours after media renewal, to 0.016 mg/L and to less than the limit of quantification (LOQ) of the analytical method, determined to be 0.013 mg/L, respectively indicating that the test item was unstable over the test duration.

Given that the toxicity cannot be attributed to a single component or mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.

CONCLUSION

Exposure of Daphnia magna to the test item gave EL50 values of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading Rate was 100 mg/L loading rate WAF.