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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

Fast Green FCF was tested in Paramecium caudate(ciliated) under static condition for 20 min. LC50 was considered to be 1000 mg/l of Fast Green FCF in Paramecium caudate(ciliated) under static condition.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

In toxicity to microorganisms study the toxic effect of fast green FCF in Paramecium caudate (ciliated) for 20 min in concentration of 1000mg/l were observed.. Fast green FCF was put in a hollow slide glass, with an equal volume of 0.04 M phosphate buffer,at pH 7.0. After 5 to 10 PC were added, to measure their survival times were. Thirty to forty PC for 0.1% concentrationwere tested, and the mean survival time and the death rate were calculated.. The death rate was defined as the percentage of deaths observed during 20 min. The LC50 was considered to be1000 mg/l of Fast Green FCF in Paramecium caudate(ciliated) under static condition.