Dihydrogen (ethyl)[4-[4-[ethyl(3-sulphonatobenzyl)amino](4-hydroxy-2-sulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene](3-sulphonatobenzyl)ammonium, disodium salt

Endpoint summary

Description of key information

Short term toxicity to fish:

A short-term toxicity test was performed with Japanece rice fish (Oryzias latipes). The fish were before the exposure assessment acclimatized during 10 days. 10 fish were tested in one exposure concentration (3000 mg/L). The test was performed at the concentration of 3000 mg/l of Fast Green FCF during 48 hours. After the exposure were number of viable individuals counted. All fish were thriving after the exposure (i.e. 100%). Hence,Fast Green FCF's LC0/NOEC was estimated to be 3000 mg/l in fish.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of Fast Green FCF,

to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 200 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The 4% inhibition concentration (IC) for the test substance fast green FCF, in Daphnia magna was determined at 200 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the above value, the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Short term toxicity to aquatic algae:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The test solution 160 mg/l was prepared by dissolving green powder in OECD growth medium. Effects on the growth rate of the organism were studied (Changable). Various concentration were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substanceFast Green FCF, in algae was determined to be 62.9 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and can be be classified as aquatic hronic 3 category as per the CLP criteria.

Additional information

Short term toxicity to fish:

A short-term toxicity test was performed with Japanece rice fish (Oryzias latipes). The fish were before the exposure assessment acclimatized during 10 days. 10 fish were tested in one exposure concentration (3000 mg/L). The test was performed at the concentration of 3000 mg/l of Fast Green FCF during 48 hours. After the exposure were number of viable individuals counted. All fish were thriving after the exposure (i.e. 100%). Hence,Fast Green FCF's LC0/NOEC was estimated to be 3000 mg/l in fish.

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity ofFast Green FCF,

to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 200 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The 4% inhibition concentration (IC) for the test substance fast green FCF, in Daphnia magna was determined at 200 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the above value, the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Short term toxicity to aquatic algae:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The test solution 160 mg/l was prepared by dissolving green powder in OECD growth medium. Effects on the growth rate of the organism were studied (Changable). Various concentration were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substanceFast Green FCF, in algae was determined to be 62.9 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and can be be classified as aquatic hronic 3 category as per the CLP criteria.