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Ecotoxicological information

Endpoint summary

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Description of key information

Short term toxicity to aquatic invertebrates

Experimental study for inhibition of the mobility of daphnids was carried out with the benzyl phenylacetate according to OECD Guideline 202 (Report no. 003/17, Abitec 2017). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 0, 5, 10, 20, 40, 80 mg/L. The test was performed under static conditions in fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance benzyl phenylacetate(102-16-9) in Daphnia magna was determined to be 60.7 mg/L for immobilisation effects.

Based on the value benzyl phenylacetate was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulations.

Toxicity to aquatic algae and cyanobacteria

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate (CAS no. 102 -16 -9) to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions  of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.

Toxicity to microorganisms

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was found to be  >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl phenylacetate.

Additional information

Short term toxicity to aquatic invertebrates

Experimental study for inhibition of the mobility of daphnids was carried out with the benzyl phenylacetate according to OECD Guideline 202 (Report no. 003/17, Abitec 2017). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 0, 5, 10, 20, 40, 80 mg/L. The test was performed under static conditions in fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance benzyl phenylacetate(102-16-9) in Daphnia magna was determined to be 60.7 mg/L for immobilisation effects.

Based on the value benzyl phenylacetate was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulations.

Toxicity to aquatic algae and cyanobacteria

Experimental key studyfor the target chemical Benzyl phenylacetate(CAS no. 102-16-9) which is supported further by experimental study for its closest structurally similar read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:

 

In an experimental key study,freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance Benzyl phenylacetate (CAS no. 102 -16 -9) according to OECD Guideline 201(Alga, Growth Inhibition Test) (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.

 

In a supporting study of read across substance 2-Phenethyl phenylacetate (CAS no. 102-20-5), the effect of read across substance 2-Phenethyl phenylacetate, CAS No. 102-20-5 was studied on the growth of fresh water green alga Chlorella vulgaris (UERL Study report, Sustainability Support Services (Europe) AB, 2015). The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 2 mg/l, 3 mg/l, 4.5 mg/l, 6.75 mg/l, 10.13 mg/l and 15.19 mg/l were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated graphically through probit analysis was observed to be 13.89 mg/l. Thus, based on this value, test chemical 2-Phenethyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.

 

Thus, based on the overall reported results for target chemical Benzyl phenylacetate (ABITEC study report, 2017) and for its read across substance (UERL study report, 2015), it can be concluded that the test substanceBenzyl phenylacetatecan be considered astoxic to aquatic organisms at environmentally relevant concentrations and can be considered to be classified inaquatic chronic category 3as per the CLP classification criteria.

Toxicity to microorganisms

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was found to be  >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl phenylacetate.