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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Remarks:
BOD5/COD
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From June 8 to 14, 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Reason / purpose:
reference to other study
Qualifier:
according to
Guideline:
ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
Deviations:
yes
Remarks:
Aerate over night after addition of the inoculum
Principles of method if other than guideline:
Internal Method: SAV 01 000 03
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: Seeding water of an urban waste water
Duration of test (contact time):
ca. 5 d
Initial conc.:
ca. 6 mg/L
Based on:
test mat.
Initial conc.:
ca. 13 mg/L
Based on:
test mat.
Initial conc.:
ca. 26 mg/L
Based on:
test mat.
Initial conc.:
ca. 51.9 mg/L
Based on:
test mat.
Initial conc.:
ca. 103.9 mg/L
Based on:
test mat.
Initial conc.:
ca. 207.7 mg/L
Based on:
test mat.
Initial conc.:
ca. 415.5 mg/L
Based on:
test mat.
Initial conc.:
ca. 830.9 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 20 ± 1 °C

Reference substance:
other: D(+) - Glucose / L-Glutamic acid
Parameter:
% degradation (O2 consumption)
Remarks on result:
not determinable
Parameter:
BOD5
Value:
ca. 4 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
ca. 0.8
Remarks on result:
other: dimensionless value
Results with reference substance:
207 mg O2/l
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
BOD5 = 4 mg O2/g
BOD5/COD= 0.008
Executive summary:

Methods:

The Biological Oxygen Demand of test substance was determined according to the ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method).

Results:

BOD5 = 4 mg O2/g

BOD5/COD= 0.008

Endpoint:
biodegradation in water: screening tests
Remarks:
BOD5/COD
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From June 6 to 7, 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Reason / purpose:
reference to other study
Qualifier:
according to
Guideline:
other: DEV DIN 38*409 - H 41-1 (1980)
Qualifier:
equivalent or similar to
Guideline:
EU Method C.6 (Degradation: Chemical Oxygen Demand)
Deviations:
yes
Remarks:
Mean of 2 determinations instead of 3
Principles of method if other than guideline:
Internal Method: SAV 02 000 02
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Duration of test (contact time):
ca. 2 h
Initial conc.:
ca. 10 other: mg
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 148 ± 3 °C
Reference substance:
other: Potassium hydrogen phthalate
Parameter:
% degradation (O2 consumption)
Remarks on result:
not determinable
Parameter:
COD
Value:
ca. 496 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
ca. 0.8 other: dimensionless value
Results with reference substance:
COD = 198 mg O2/l
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
COD = 496 mg O2/g
BOD5/COD = 0.008
Executive summary:

Methods:

The Chemical Oxygen Demand of test substance was determined according to the DEV DIN 38*409 - H 41-1 (1980), similar to EU Method C.6 (Degradation: Chemical Oxygen Demand).

Results:

COD = 496 mg O2/g

BOD5/COD = 0.008

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From April 20 to May 18, 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: EEC-Directive, L133 (pp. 99-105) Volume 31, May 88.
Deviations:
no
Principles of method if other than guideline:
Internal method: SAV:13 000 02
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: Sludge of a biological sewage treatment plant
Details on inoculum:
- Source of inoculum: Sludge of a biological sewage treatment plant
- Laboratory culture: (ARA-Reinach) collected on 19.4.94
- Initial cell/biomass concentration: 930 mg/l
Duration of test (contact time):
ca. 28 d
Initial conc.:
ca. 150 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.
- Test temperature: 22.0 ± 3°C
- Lighting: indirect daylight
- Estimation Technique: TOC/DOC Analyzer/ SHIMADZU TOC-5000

Reference substance:
diethylene glycol
Parameter:
% degradation (DOC removal)
Value:
ca. 22.9
Sampling time:
28 d
Parameter:
% degradation (DOC removal)
Value:
ca. 25.3
Sampling time:
3 h
Remarks on result:
other: adsorption
Results with reference substance:
bioelimination of the reference substance after 14 days is : 99.7 %
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Bioelimination of test substance measured as DOC after 28 days corrected by the blank control is: 22.9 %
Adsorption after 3 hours: 25.3 %
Executive summary:

Methods:

The inherent biodegradability of test substance was determined in a 28 days Zahn-Wellens -test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1981.

 

Results:

Bioelimination of test substance measured as DOC after 28 days corrected by the blank control is: 22.9 %.

Adsorption after 3 hours: 25.3 %

Description of key information

DOC removal (28 d) = 22.9 %; adsorption after 3h = 25.3 %

BOD5/COD= 0.008  

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable

Additional information

No studies on the "Biodegradation in water" are available for the substance in itself.

Nevertheless studies have been conducted with an analogue molecule (Similar Substance 01). Further information are reported in the Read Across justification attached to section 13.

Different available tests may be valuable that give information on biodegradability. Missing the Ready biodegradability test.

 

Inherent biodegradability 

The inherent biodegradability was determined in a Zahn-Wellens, test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice , regulation of Switzerland. The test substance and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC.

The biodegradation of the test substance after 28 days was 22.9 %, with adsorption after 3 h of 25.3 %. The biodegradation of the reference substance after 9 days was 99.7 %.

 

BOD5/COD ratio

Biological Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) are the most commonly used parameters for the characterization of the biodegradability capacity.

COD results are typically higher than BOD5 values, and the ratio between vary depending on the characteristics of the substrate. This ratio has been commonly used as an indicator for biodegradation capacity, the "Biodegradability index" (B.I.).

As indicated in ECHA Guidance requirement R.7B, the BOD5 test is a traditional biodegradation test that is now replaced by the ready biodegradability tests. Therefore, this test should not be performed today for assessment of the ready biodegradability of substances.

Older test data may, however, be used when no other degradability data are available.

Studies on BOD5 and COD are available for this substance, therefore they have been used together with the inherent biodegradability OECD 302.B, in the weight of evidence approach for this substance.

COD was measured according to Method A-16. Details of the method have been not reported. The COD measured was 496 mg/g O2.

BOD5 was measured according to Method A-15. Details of the method have been not reported. The BOD5 measured was 4 mg/g O2, at concentration of 100 mg/L.

The ratio BOD5/COD is 0.008.

This result is in line with the BOD5/COD value calculated in other tests reported in the file attached below.

Based on the rests of the considered tests, the substance is expected to be Not Readily Biodegradable