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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EL50 (invertebrates) = 5.74 (C.I. 4.94 - 6.68) mg/L ; WAF loading 48hour-freshwater, OECD TG 202, NOACK Laboratorien GmbH 2016

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
5.74 mg/L

Additional information

The acute toxicity to Daphnia magna was carried out according to OECD TG 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. The study was conducted in a closed system (sealed glass flasks) without headspace under semi-static conditions over a period of 48 hours with five Water Accommodated Fractions (WAF) of the test item with loading levels in the range of 0.625 to 10.0 mg/L prepared in a geometric series with a separation factor of 2.0. Twenty daphnids were exposed to each loading level and a control. The total carbon (TOC, according to DIN EN 1484) was measured at the start of the exposure intervals (0 and 24 hours) in all loading levels and in the control. Analysis of the TOC is the most suitable method for the analytical monitoring of poorly soluble UVCB substances. Per definition of the WAF, all terms related to concentration levels have to be given as loading levels because partly dissolved compounds and mixtures cannot be related to concentrations. Therefore, the effect levels given were based on the nominal loading levels of the test item. The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 hours) and at the end of the exposure intervals (24 and 48 hours), were within the acceptable limits. The validity criteria of the test guideline were fulfilled. The 48h-EL50 was 5.74 mg/L (C.I: 4.94 – 6.68 mg/L) based on nominal loadings of the test item into the test system.