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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Authoritative database
Justification for type of information:
Data from ecotox database.
Qualifier:
according to
Guideline:
other: as below
Principles of method if other than guideline:
Toxic effects of test chemical were evaluated in Daphnia magna in a 1 day study.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water Flea
- Age at study initiation (mean and range, SD): <=24 h
Test type:
static
Water media type:
freshwater
Total exposure duration:
1 d
pH:
8
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
750 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
behaviour
Validity criteria fulfilled:
not specified
Conclusions:
EC50 value for test chemical in Daphnia magna was determined to be 750 mg/l in a 24 hrs study.
Executive summary:

In short term toxicity to aquatic invertebrate study for test chemical in Daphnia magna was observed for 24 hrs.Less than 24 hrs 5 daphnia were used in fresh water and static condition.EC 50 was observed at range concentration of 750 mg/l on the basis of behaviour. Based on the value, test chemical was considered to be non-toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

Description of key information

Short term toxicity to aquatic invertebrate:

Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 200 mg/l was prepared by dissolving colorless  liquid in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 10, 20, 40, 80, 160 mg/L. Potassium dichromate (K2Cr2O7) was used as a positive reference substance . Effects on immobilization were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance ethyl butyrate in Daphnia magna was determined to be 116.6 mg/L for immobilization effects. This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and can not be classified as toxic as per the CLP criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
116.6 mg/L

Additional information

Toxicity of aquatic invertebrate was evaluated based on the two experimental data which were performed according to OECD guideline and along with that data from authoritive data base and secondary sources were also discussed as below:

Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 200 mg/l was prepared by dissolving colorless liquid in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 10, 20, 40, 80, 160 mg/L. Potassium dichromate (K2Cr2O7) was used as a positive reference substance. Effects on immobilization were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance ethyl butyrate in Daphnia magna was determined to be 116.6 mg/L for immobilization effects. This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and can not be classified as toxic as per the CLP criteria.

 

First study was supported by the second. Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/l. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/l . After 48 hours of exposure to test item to various nominal test concentrations, EC50 was determine to be > 100 mg/l . Based on the EC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

 

Similar short term toxicity study of test material was carried out on the Daphnia magna and was observed for 24 hrs. Less than 24 hrs 5 daphnia were used in fresh water and static condition at 20 -22 degree C temperatures. 50% mortality (LC 50) was observed at range concentration of 755 mg/l. Based on the value, ethyl butyrate was considered to be non-toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

 

In short term toxicity to aquatic invertebrate study for Ethylbutyrate in Daphnia magna was observed for 24 hrs.Less than 24 hrs 5 daphnia were used in fresh water and static condition.EC 50 was observed at range concentration of 750 mg/l on the basis of behaviour. Based on the value, ethyl butyrate was considered to be non-toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

 

Based on the above data for test chemical it can be concluded that test chemical is non toxic to aquatic invertebrate and can be considered to be not classified as per CLP criteria.