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Diss Factsheets
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EC number: 200-665-9 | CAS number: 67-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of various test chemicals
- Justification for type of information:
- Experimental data of various test chemicals is from collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE was prepared for determination of toxicity of test chemical on microorganisms.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- WoE 2: Tetrahymena pyriformis was preserved in a sterile medium of 2% proteose peptone at 20°C which was renewed at 2-4 week intervals.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 24 d
- Remarks on exposure duration:
- WoE 3: 17 hrs
- Test temperature:
- 2: 30 deg C
- Details on test conditions:
- 2: The stock solution of the chemical was added to the sterile medium to provide a concentration ratio of 1.8 in 10ml of 2% proteose peptone. The solutions were then inoculated with 0.2 ml of pre-cultured T. pyriformis and cultivated at 30°C for 24 h without agitation. The number of cells was then counted.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Population growth rate
- Remarks on result:
- other: 2nd study
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 3rd study
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 2nd study: The median effective concentration (EC50) value of test chemical in tetrahymena pyriformis was observed to be 32000 mg/L on the basis of population growth rate effect in a 24 hours of exposure.
3rd study: The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L. - Executive summary:
Based on the effect observation of test chemical on growth of microorganisms, studies have been reviewed and summarized as mention below:
In this study from peer reviewed journal, a test was developed using Tetrahymena pyriformis in order to determine the toxicity of test chemical. Pre-cultured T. pyriformis was exposed for 24 h at 30°C to various concentrations, and the number of T. pyriformis surviving were then counted. The concentration of the chemical, at which the proliferation of T. pyriformis was restricted to one-half of the blank test (EC50), was determined. The median effective concentration (EC50) value of test chemical in tetrahymena pyriformis was observed to be 32000 mg/L on the basis of population growth rate effect in a 24 hours of exposure. Thus, it can be concluded that the test substance does not exhibit any toxicity effect to microorganism (tetrahymena pyriformis).
Similarly in the next study from peer reviewed journal toxicity on the bacterial growth were observed for 17 hrs. Short term toxicity of chemical was studied on the growth of bacteria. The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L.
Reference
Description of key information
2nd study: The median effective concentration (EC50) value of test chemical in tetrahymena pyriformis was observed to be 32000 mg/L on the basis of population growth rate effect in a 24 hours of exposure.
3rd study: The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 32 000 mg/L
Additional information
Based on the effect observation of read across chemical selected on the basis of functional similarity with the test chemical on the growth of microorganisms, studies have been reviewed and summarized as mention below:
In this study from peer reviewed journal, a test was developed using Tetrahymena pyriformis in order to determine the toxicity of test chemical. Pre-cultured T. pyriformis was exposed for 24 h at 30°C to various concentrations, and the number of T. pyriformis surviving were then counted. The concentration of the chemical, at which the proliferation of T. pyriformis was restricted to one-half of the blank test (EC50), was determined. The median effective concentration (EC50) value of test chemical in tetrahymena pyriformis was observed to be 32000 mg/L on the basis of population growth rate effect in a 24 hours of exposure. Thus, it can be concluded that the test substance does not exhibit any toxicity effect to microorganism (tetrahymena pyriformis).
Similarly in the next study from peer reviewed journal toxicity on the bacterial growth were observed for 17 hrs. Short term toxicity of chemical was studied on the growth of bacteria. The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L.
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