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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
The main objective of this study is to evaluate the effect of test chemical on the daphnia magna
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
the test solution was prepared by dissolving 500 mg of the test substance in 500.ml of ADaM’s media
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
150.5 mg of CaCO3
Test temperature:
20±2
pH:
7.01
Nominal and measured concentrations:
nominal cocncentration: 6.25 mg/L,12.5 mg/L, 25 mg/L, 50 mg/L, and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Immobility

Nominal

Concentration

(mg/l)

Number of

Daphnids

                                                     Immobility

0 h

24h

48h

Control

10

-

-

-

6.25

10

-

-

5

 12.5  10  -  -  10
 25  10  -  10  10
 50  10  -  10  10
 100  10  -  10  10

   

Ph and oxygen content:

Nominal Concentration (mg/l)

Number of daphnids

pH(mg/L)

0h

48h

Control

10

7.30

7.40

6.25

10

7.35

7.16

12.5

10

7.39

7.14

25

10

7.41

7.17

50

10

7.45

7.20

100

10

7.48

7.21

Nominal Concentration (mg/l)

Number of daphnids

DO(mg/L)

0h

48h

Control

10

7.01

6.9

6.25

10

7.09

6.89

12.5

10

7.09

6.89

25

10

7.09

6.89

50

10

7.09

6.86

100

10

7.09

6.85

Test Temperature:

Nominal Concentration (mg/l)

Number of daphnids

Temparature

0h

48h

Control

10

21.5

20.9

6.25

10

21.5

20.9

12.5

10

21.5

20.9

25

10

21.5

20.9

50

10

21.5

20.9

100

10

21.5

20.9

Validity criteria fulfilled:
yes
Remarks:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
Conclusions:
The median effective concentration of test chemical to daphnia magna was determined to be 6.25 mg/L.
Executive summary:

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Description of key information

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.25 mg/L

Additional information

In the following information from experimental studies report and different peer reviewed journal on short term toxicity testing to aquatic invertabrates for target chemical is summarized:

Acute Immobilization Test of daphnia magna (performed according to OECD Guideline 203) was conducted to assess the toxic effect of test chemical. The test was performed in a static exposure conditions for the period of 48 hours. Test solution was prepared by dissolving the test chemical in the ADaMs medium, and Daphnia magna was treated with different concentrations (6.25, 12.5, 25, 50, and 100 mg/L), along with the control (daphnia exposed without test chemical). Based on the immobilization effect of test chemical to daphnia magna (n=10, per replicate). The median lethal concentration was evaluated. Results conclude that the test chemical has caused the 100% immobilisation of daphnia at all the concentration > 6.25 mg/L. The obtained data from the report determines that EC50 concentration is 6.25 mg/L. From the obtained value the test chemical can be considered to toxic in nature and can be classified into aquatic chronic 2 category.

Short term toxicity test was performed on Daphnia magna for test chemical for 24 hrs under static conditions. No effect on mortality of fish was observed, Therefore, the LC0 value of test chemical to Daphnia magna was determined to be 1 mg/l.

Short term toxicity test was performed on Daphnia magna for 24 hrs under static condition .Lethal concentration to 100% of test organisms was observed, therefore theLC100 of test chemical to Daphnia magna was determined to be 100mg/l.

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

The stock solution 20.0 mg/L was prepared by dissolving white powder in reconstituted water. The solution was kept in ultrasonic bath for 20 min. The test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water.0.05,0.10,0.20,0.40,0.80 mg/L concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 0.09 mg/L (95 % CI :0.08-0.11) on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute 1 category as per the CLP classification criteria.

Even though varied results for short term toxicity of aquatic invertebrate were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is toxic and can be classified as aquatic chronic 2.