5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: 40 CFR, Part 163, Section 163.81-5, Federal Register, August 22, 1978.

Deviations:

not specified

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

Exposure duration of 24 hours

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

5,5'-dithiodi-1,3,4-thiodiazole-2(3H)-thione

IUPAC Name:

5,5'-dithiodi-1,3,4-thiodiazole-2(3H)-thione

Constituent 2

Reference substance name:

5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione

EC Number:

276-763-0

EC Name:

5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione

Cas Number:

72676-55-2

Molecular formula:

C4-H2-N4-S6

IUPAC Name:

5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione

Test material form:

solid: particulate/powder

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

Animals were housed and maintained according to the Animal Welfare Act (Pub. L-94-279) 9 C.F.R. Part 3.

Animal husbandry conditions:
Temperature - 70ºF ±5ºF
Relative humidity - 45% ± 5%
Light - 12 hour light/dark cycle
Diet - Wayne 15% rabbit ration and tap water provided ad libitum.
Caging - Stainless steel with elevated wire mesh flooring 1 rabbit per cage.
Weight - weighing between 2.0 and 3.0 kg.
Age - approximately 9-10 weeks of age.
Acclimatisation - for at least 5 days prior to study.

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: abraded or intact

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

A 0.5 gram sample of 5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione was dosed as supplied and applied to two abraded and two intact skin sites on the same animal.

Duration of treatment / exposure:

Exposure of the material over a period of 24 hours, at the end of which the sites were wiped but not washed and the treated areas examined.

Observation period:

Readings were taken after 24 hours and again made after 72 hours.
The Draize method of scoring was employed. All lesions observed were recorded.

Number of animals:

six

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Irritant / corrosive response data:

The Primary Irritation score was 0 and the average combined irritation score was 0 for Erythema and Eschar formation and Edema formation in all animals over 72 hours on both abraded and intact skin.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Not skin irritating

Conclusions:

5,5'-dithiodi-1,3,4-thiadiazole-2(3H)-thione is not classified as a primary skin irritant to the skin of albino rabbits.