Reaction mass of 1,2-Diacetoxy-3-butene and 1,2-Dihydroxy-3-butene, monoacetate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

445-630-0

EC Name:

-

Molecular formula:

Unspecified

IUPAC Name:

1-(acetyloxy)but-3-en-2-yl acetate; 2-hydroxybut-3-en-1-yl acetate

Details on test material:

Test substance: Vinylglykoldiacetat
- batch No.: Kanister 1, 2, 4, and 5

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

- Dilution water chemistry: according to OECD 201
- Preparation of the test media: The test medium of the highest test concentration (nominal 111,3 mg/l) in test medium was prepared using a 10 min stirring period at 20 +/- 2 °C. Then this test medium was serially diluted to obtain the desired test concentrations. A concentrated stock solution of the test substance in test medium (nominal 1000 mg/l) was prepared using Cremophor (100 mg/l) as a solvent. Then, serial dilutions of this stock solution were prepared to obtain the desired test concentrations. The maximum solvent concentration in the test was 10 mg/l.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

- Test organisms: green alage
- Test species: Desmodesmus subspicatus (formerly known as Scenedesmus subspicatus), Chodat, Strain SAG 86.81
- Supplier: Collection of algae cultures, University of Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Details on test conditions:

Test conditions
- Exposure vessels: 250 ml Erlenmeyer flask with glass plugs
- Test volume: ca. 300 ml
- Light conditions: continuous white light Light intensity: about 60 - 120 microE/(m2 * s) at 400 - 700 nm
- Number of replicates: 3
- Initial cell number: about 10000 cells/ml
- Test temperature during the test: 23 +/- 2 °C
- pH values during the test: not indicated
- Measurement: in vivon chlorophyll-a-fluorescence after 0, 24, 48 and 72 hours.
- Nominal test concentrations: ranging from 1.56 to 100 mg/l, using a spacing factor of 2.
- A medium control, was tested in parallel.

- Chemical analysis: At test start and test end, samples from all test concentrations the control were subjected chemical analysis using a HPLC-method.
- Statistics: The EC10, EC50 and EC90 values were determined using probit analysis.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 71.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

30.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

35.7 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

15.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 71.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Cell densities after x hours of exposure [*10E+04] 

Nominal test  / Exposure time [h]
concentration /
(mg/l)        /  0 / 24 /  48 /  72 /  
Control       / 2.8 / 12.9 / 57.6 / 151.7 /
1.56          / 2.9 / 13.5 / 62.4 / 154.2 /
3.13          / 2.9 / 11.7 / 58.6 / 148.3 /
6.25          / 2.9 / 11.7 / 53.1 / 153.2 /
12.5          / 2.8 / 11.1 / 52.1 / 163.7 /
25            / 2.9 /  9.9 / 42.0 / 146.3 /
50.0          / 2.9 /  8.1 / 30.7 / 124.9 /
100.0         / 2.9 /  5.9 / 16.8 /  45.5 /

% inhibition of growth rate and biomass after 72 h
             growth rate u / biomass b / 
Control       -    /   -       /
1.56          -0.1 /  -4.7     /
3.13           0.7 /   1.4     /
6.25           0.4 /   3.7     /
12.5          -2.0 /   0.9     /
25.0           1.3 /  15.4     /
50.0           5.5 /  32.5     /
100.0         30.5 /  72.4     / 

Analytically determined recovery rate of the test substance
The analytically determined recovery rate of the test concentrations  ranged from 163.5 % to n.d. at test start and test end, respectively.

Therefore, all biological results were referred to mean measured test  concentrations. However, the biological results refer to test substance  concentrations and were not corrected to the content of the active  ingredient (about 75 %)

EC-values after 72 hours:
growth rate
EuC10: 35.7 mg/l
EuC50: > 71.2 mg/l
NOEC: 15.3 mg/l
LOEC: 30.6 mg/l

biomass
EbC10: 11.9 mg/l
EbC50: 44.3 mg/l
NOEC: 4.1 mg/l
LOEC: 15.3 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes