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EC number: 201-923-3 | CAS number: 89-61-2
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- The Mutagenicity of Dinitrochlorobenzene
- Author:
- Homer S. Black
- Year:
- 1 985
- Bibliographic source:
- Arch Dermatol Vol 121 March 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as per mentioned below
- Principles of method if other than guideline:
- A sample of 2, 5 dinitrochlorobenzene, determined to be have purity greater than 98%, was tested in the Salmonella typhimurium plate assay.
- GLP compliance:
- no
- Type of assay:
- bacterial gene mutation assay
Test material
- Reference substance name:
- 89-61-2.
- IUPAC Name:
- 89-61-2.
- Reference substance name:
- 1,4-dichloro-2-nitrobenzene
- EC Number:
- 201-923-3
- EC Name:
- 1,4-dichloro-2-nitrobenzene
- Cas Number:
- 89-61-2
- Molecular formula:
- C6H3Cl2NO2
- IUPAC Name:
- 1,4-dichloro-2-nitrobenzene
- Reference substance name:
- 1,4-dichloro-2- nitrobenzene
- IUPAC Name:
- 1,4-dichloro-2- nitrobenzene
- Details on test material:
- Details on test material
- Name of test material (as cited in study report): 1,4-dichloro-2- nitrobenzene
- Molecular formula (if other than submission substance): C6-H3-Cl2-N-O2
- Molecular weight (if other than submission substance): 192.001 g/mol
- Substance type: Organic
- Physical state: Solid
Purity: > 98%
- Impurities (identity and concentrations): No data available
Constituent 1
Constituent 2
Constituent 3
Method
- Target gene:
- Reverse mutation assay
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not specified
- Metabolic activation system:
- no data
- Test concentrations with justification for top dose:
- 1 µg/plate
- Vehicle / solvent:
- Vehicle
- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: No data available
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- no
- Positive control substance:
- sodium azide
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available
NUMBER OF REPLICATIONS: Four
NUMBER OF CELLS EVALUATED: No data available
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: Colony counts were determined with an automatic colony counter (3M Model 620). - Evaluation criteria:
- His+ Revertants/Plate was observed
- Statistics:
- The Student t test was employed to
statistically evaluate the mutation frequencies; Mean ± SEM
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- not specified
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No data available
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
The gene toxicity in vitro response for the test compound 2,5 dinitrochlorobenzene in the Salmonella typhimurium tester strain TA-100 is positive. - Executive summary:
2, 5 dinitrochlorobenzene was used as a test compound to evaluate its toxic nature in theSalmonella typhimuriumtester strain TA-100. The mutagenicity assay was performed as per the Ames et al without any modifications.
In accordance with the marked mutagenic response noted, 2, 5 dinitrochlorobenzene is mutagenic in vitro.
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