Nonan-2-one

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Toxicity to micro-organisms study was conducted on Tetrahymena pyriformis for 48 hrs

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2-Nonanone
- Molecular formula (if other than submission substance): C9H18O
- Molecular weight (if other than submission substance): 142.2402 g/mol
- Smiles notation (if other than submission substance): C(CCCCC)CC(C)=O
- InChI: 1S/C9H18O/c1-3-4-5-6-7-8-9(2)10/h3-8H2,1-2H3
- Substance type: Organic
- Physical state: Liquid

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 6 or more different test substance conc. was used for the study, exact conc. of test chemical was not reported.

- Sampling method: Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l.

- Sample storage conditions before analysis: No data available

Vehicle:

yes

Details on test solutions:

Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO)

Test organisms (species):

Tetrahymena pyriformis

Details on inoculum:

No data available

Test type:

not specified

Water media type:

not specified

Total exposure duration:

48 h

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

No data available

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

TEST SYSTEM
- Test vessel: No data available
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: No data available
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): Duplicates
- No. of vessels per control (replicates): Duplicates
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Population density was measured spectrophotometrically at 540 nm.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates.
- Test concentrations: 6 or more different test substance conc. was used for the study, exact conc. of test chemical was not reported.
- Results used to determine the conditions for the definitive study: No data available

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

other: IGC50

Effect conc.:

449 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software with Y as the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.

Table: Log IGC50^-1and IGC50 (mM) value of test substance to Tetrahymena pyriformis strain was given.

 

Chemical	Log IGC50^-1	IGC50 (mM)
2-nonanone	0.50	3.163

Validity criteria fulfilled:

not specified

Conclusions:

Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs. Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.

Executive summary:

Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs.

 

Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alterTetrahymena population growth.

 

Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.

 

After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.

 

The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software withYas the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.

 

Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.

Description of key information

Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs.

 

Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alterTetrahymenapopulation growth.

 

Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.

 

After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.

 

The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software withYas the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.

 

Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.

Additional information

Four different experimental studies from peer reviewed journal for toxicity to micro organism endpoint for target chemical 2-Nonanone (Cas no. 821-55-6) are summarized as followed:

First experimental study suggest toxicity to micro-organisms study was conducted on Tetrahymena pyriformis for 48 hrs.Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alter Tetrahymena population growth.Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.

After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software with as the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million. Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.

Another experimental study indicates the effects of 2 nonanone on Tetrahymena pyriformis was conducted under static condition. The test was allowed to run through 8-9 cell cycles. Semi defined proteose-peptone-based medium were inoculated to a density of 2500 cells/ml with log-growth-phase ciliates in the presence of the test material in DMSO. The test material was evaluated in each of three replicates at 6-8 different concentrations. Controls were inoculated with T. pyriformis in the absence of test material. Following incubation at 27 C, the population density of T. pyriformis was measured at 540 nM. The 50% growth inhibitory concentration was determined from probit analysis of absorbance values andconcentrations.IGC50 was found to be 33.26 mg/L under static condition for 2-Nonanone for 50% growth inhibition of Tetrahymena pyriformis.

Another experiment suggest In Toxicity to microorganisms study for 2-Nonanone was observed for Penicillium digitatum FRR 1562 and Colletotrichum musae DAR 24962.The MIC of each of the volatiles was evaluated and compound was classified as germicidal or germistatic in its effect on decay microorganisms. Agermicidal effect is the death of a microorganism, whereas a germ static effect is the inhibition of microbial replication). The agar disks of decay microorganisms which failed to grow due to the MIC of the compound were transferred onto new agar media free from the tested volatile and incubated for a further 5 days at 25 °C.The MIC observed with respect to 2 nonanone in Penicillium digitatum FRR 1562 was found to be 4.82 mmol/dish and for Colletotrichum musae DAR 24962 was found to be 3.58 mmol/dish.

And last experimental result suggest The 10 days toxicity study was conducted to evaluated to inhibitory effects of 2 nonanone.Erwinia carotovora UNSW 031700 was used as a test organism exposed to the concentration of 1.5ml/dish The NOEC was found to be 1.5m/dish for Erwinia carotovora.

Above all experimental values for Inhibition growth concentration (IGC50) and minimum inhibition concentration (MIC) conclude the target chemical 2-Nonanone (Cas no. 821-55-6) has no concern for toxicity to micro organism for acute exposure.