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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October - November 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-guideline study
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentration: 100 mg/l
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Strain: HAMILTON BUCHANAN
- Source: Charles River (Sulzfeld)
- Age at study initiation: about 6 month
- Length at study initiation: 2.5 - 3.5 cm


ACCLIMATION
- Medical treatment: none
- Keeping water: synthetic fresh water in accordance with ISO
- Mortality while kept: < 2%
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
No mortalities or sub-lethal effects of exposure were observed.
Hardness:
14.2 °dH
Test temperature:
22.1 - 22.4 °C
pH:
7.4 - 7.6
Dissolved oxygen:
8.0 - 9.3 mg / l
Salinity:
no data reported.
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 x 135 x 200 mm
- Fill volume: 5 l test medium
- Aeration: ventilated
- Type: static

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water in accordance with ISO

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark

TEST CONCENTRATIONS
- Test concentration: 100 mg / l
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: behaviour, body length and weight
Details on results:
No mortalities or sub-lethal effects of exposure were observed for the test concentration of 100 mg / l.
Reported statistics and error estimates:
The above result refers to nominal concentration as a direct relationship between measured TOC values and C-containing components of the test substance (= benzene amine) which cannot be assessed quantitatively. On the other hand, in aqueous solutions benzene amine reacts to benzene aldehyde. As both components have nearly identical molecular weights, the TOC values, fairly constant throughout the test period, reflect rather constant exposure rates of the sum of both chemical bodies, benzene amine and benzene aldehyde, respectively.

Visible effects on appearance and behaviour of fish

Control substance:

 after 2 hours  10 animals normal swimming action
 after 24 hours  10 animals normal swimming action
 after 48 hours  10 animals normal swimming action
 after 72 hours  10 animals normal swimming action
 after 96 hours  10 animals normal swimming action

Test substance, 100 mg/l:

 after 2 hours  10 animals normal swimming action
 after 24 hours  10 animals normal swimming action
 after 48 hours  10 animals normal swimming action
 after 72 hours  10 animals normal swimming action
 after 96 hours  10 animals normal swimming action

Body length and weight after sacrificing at end of study

Test fish      Body length [cm] at control and test substance
   Control  100 mg test substance / l
 1 2.8  2.6
 2  3.1  3.2
 3  3.0  3.5
 4  3.1  3.3
 5  2.5  2.6
 6  3.0  3.4
 7  2.5  2.9
 8  2.9  2.9
 9  2.8  3.5
 10  3.3  3.5
 fresh weight [g] of 10 fishes  2.18  2.7
Validity criteria fulfilled:
yes
Conclusions:
The study is regarded as a valid guideline study with GLP compliance. According to the reported LC 100 value (> 100 mg/L) the test substance is not classified as toxic to fish.
Executive summary:

The acute toxicity of the substance (benzylamine)trifluoroborone was investigated according to EU Method C.1 in compliance with GLP. Brachydanio rerio (Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 22.1 °C - 22.4 °C (control: 21.9 °C - 22.4 °C) and a pH range of 7.4 - 7.6 (control: 7.5 - 7.7) and a substance concentrations of 100 mg/L. Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As result a LC 100 of > 100 mg/L is reported.

Description of key information

EU Method C.1, GLP, Brachydanio rerio (Zebra fish), static freshwater, LC 100 > 100 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The acute toxicity of the substance (benzylamine)trifluoroborone was investigated according to EU Method C.1 in compliance with GLP.Brachydanio rerio(Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 22.1 °C - 22.4 °C (control: 21.9 °C - 22.4 °C) and a pH range of 7.4 - 7.6 (control: 7.5 - 7.7) and a substance concentrations of 100 mg/L. Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As result a LC 100 of > 100 mg/L is reported.