(benzylamine)trifluoroboron

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1996 - January 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well documented GLP-guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

Effluent of laboratory scale unit receiving sewage from the south Wupper area water authority (predominantly domestic sewage).

Duration of test (contact time):

28 d

Initial conc.:

18.2 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 22 +- 2 °C
- Concentration of stock solution of the sample: 1 g/l
- Initial concentration of the reference substance: 17.5 mg/l DOC
- Continuous darkness: yes

CONTROL AND BLANK SYSTEM
- Toxicity control: test and reference substance plus inoculum

Reference substance:

aniline

Preliminary study:

No information about a preliminary study are available.

Test performance:

The used concentrations of the test substance did not show toxic effects to bacteria.
By technical reasons no DOC-determination has been carried out at day 27. Therefore an additional DOC-determination has been carried out at day 29.

Parameter:

% degradation (DOC removal)

Value:

92

Sampling time:

7 d

Parameter:

% degradation (DOC removal)

Value:

94

Sampling time:

14 d

Parameter:

% degradation (DOC removal)

Value:

94

Sampling time:

21 d

Parameter:

% degradation (DOC removal)

Value:

96

Sampling time:

28 d

Details on results:

The test substance is considered to be "Readily Biodegradable".

Results with reference substance:

In parallel preparations with the reference substance aniline a degradation of 92 % was achieved within 14 days. All validity criteria of the test method were met.

test substance	% degradation after n days
flask no.	0 h	7 d	14 d	21 d	28 d	29 d
8	0	88	90	95	98	99
9	0	96	97	92	94	98
mean	0	92	94	94	96	99
reference substance	% degradation after n days
flask no.	0 h	7 d	14 d	21 d	28 d	29 d
3	0	100	89	95	97	96
4	0	98	95	94	95	96
mean	0	99	92	95	96	96
toxicity control	% degradation after n days
flask no.	0 h	7 d	14 d	21 d	28 d	29 d
10	0	84	92	94	96	97

Validity criteria fulfilled:

yes

Remarks:

All validity criteria of the test method were met.

Interpretation of results:

readily biodegradable

Conclusions:

Within 28 days, a degradation of 96 % was determined for Lekutherm-Beschleuniger KU 6519 Neu. Lekutherm-Beschleuniger KU 6519 Neu has to be classified as "Readily Biodegradable".

Executive summary:

The biodegradability of the substance Lekutherm-Beschleuniger KU 6519 Neu was determined according to EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test). The studiy was conducted in compliance with the Principles of Good Laboratory Practice (GLP).

The test substance is suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms and incubated for 28 days in the dark at 22 +- 2 °C. During this period, the biodegradation of the test substance is determined on the basis of the reduction in DOC. Within 28 days, a degradation of 96 % was determined for Lekutherm-Beschleuniger KU 6519 Neu. Lekutherm-Beschleuniger KU 6519 Neu has to be classified as "Readily Biodegradable".

In parallel preparations with the reference substance aniline a degradation of 92% was achieved within 14 days. All validity criteria of the test method were met.

Description of key information

Within 28 days, a degradation of 96 % was determined for Lekutherm-Beschleuniger KU 6519 Neu. Lekutherm-Beschleuniger KU 6519 Neu has to be classified as "Readily Biodegradable".

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

The biodegradability of the substance Lekutherm-Beschleuniger KU 6519 Neu was determined according to EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test). The studiy was conducted in compliance with the Principles of Good Laboratory Practice (GLP).

The test substance is suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms and incubated for 28 days in the dark at 22 +- 2 °C. During this period, the biodegradation of the test substance is determined on the basis of the reduction in DOC. Within 28 days, a degradation of 96 % was determined for Lekutherm-Beschleuniger KU 6519 Neu. Lekutherm-Beschleuniger KU 6519 Neu has to be classified as "Readily Biodegradable".

In parallel preparations with the reference substance aniline a degradation of 92% was achieved within 14 days. All validity criteria of the test method were met.