Registration Dossier
Registration Dossier
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EC number: 250-333-2 | CAS number: 30772-79-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
A study was performed to assess the effect of the test material on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD TG No 201. Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed for 72 hours to 8 concentrations of the test substance, prepared from a stock solution of the test material prepared at 100 mg/L (three replicate flasks per concentration), under constant illumination and shaking at a temperature of 21 - 23°C.
Samples were taken from all treatments at t = 0 , 24, 48 and 72 h and analysed with a validated GC-FID method. At the start of the test, the actual test concentrations were generally in agreement with nominal. The concentrations decreased significantly during the test period. The decreases at the lower concentrations were more significant than those at the higher concentrations. The decrease in the solution incubated without algae (3.2 mg/L) was also less significant than the decrease in the same solution with algae indicative for an adsorptive effect.
Based on the fact that the actual test concentrations decreased to less than 80% of the nominal test concentrations, the geometric mean concentrations were used for calculation of effect concentrations.
Nominal concentrations of 0.032, 0.10, 0.32, 1.0, 3.2, 10, 32 and 100 mg/L corresponded with geometric mean measured concentrations of0.025, 0.028, 0.047, 0.17, 1.0, 6.9, 20 and 66 mg/L, respectively.
Statistically significant inhibition of growth rate was found at geometric mean measured concentrations of 0.32 mg/L and higher, however, the effect observed at 1.0 mg/L was considered biologically insignificant (<10%). Therefore this value is used as NOEC.
The ErC10 and ErC50 based on geometric mean measured concentrations were 4.7 and 9.5 mg/L, respectively
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 9.5 mg/L
- EC10 or NOEC for freshwater algae:
- 4.7 mg/L
Additional information
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