Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets national standard methods with accptable restrictions

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987
Reference Type:
other: recalculation
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: original cited: "Algae inhibition test supported by the UBA (German environmental government agency) " ressembling DIN 38412/9
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triphenylphophinoxid
- Analytical purity: not stated

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae, (Scenedesmus subspicatus)
- Strain: SAG 86.81
ACCLIMATION
Culturing volume: 100 ml

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20 °C
Nominal and measured concentrations:
Nominal concentrations: 0.976, 1.95, 3.9, 7.81, 15.62, 31.25, 62.5, 125 mg/L. A control was tested in paralell.
The stock solution was hardly soluble
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Type (delete if not applicable): open
- Initial cells density: Initial count of algae cells/ml: 10000

GROWTH MEDIUM
- Standard medium used: yes; OECD Medium
Temperature: 20 °C
Culturing volume: 10 ml


TEST MEDIUM / WATER PARAMETERS
Test substance preparation: heating
- Culture medium different from test medium: no


Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
3.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
6.04 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
20.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
9.81 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
14.366 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
29.644 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
7.812 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
3.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Recalculation (ToxRatPro v2.09)
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
7.599 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: cell denstity
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
18.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: cell density
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
56.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: cell density
Details on results:
- Exponential growth in the control: no

Applicant's summary and conclusion