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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16.05 - 20.05. 1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study according to national standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: to German national standard DIN 38 412, part L15.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triphenylphosphinoxid roh
- Purity: about 80 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Subsequently the fish were placed into the test aquaria.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe
- Strain: Leuciscus idus L. (golden variety)
- Source: Fish farm Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 7.8 cm (range: 6.6 - 9.1 cm)
- Weight at study initiation (mean and range, SD): 4.7 g (range: 3.1 - 6.7 g)
- Method of breeding: Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system
Duration of housing: about 6 months
Mortality during the last 2 weeks of housing: 0.9 %
Medical treatment: Twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride


ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Growing feed F/B 50,
- Feeding frequency: ad libitum
- Mortality during the acclimation period: 0 %
Withdrawal of food: 1 day before and during exposure.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/l
Test temperature:
20 ± 1 °C
pH:
about 8.0
Dissolved oxygen:
7.1 - 8.8 mg/l
Nominal and measured concentrations:
Nominal concentrations: 0, 10, 21.5, 46.4, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.7 g fish/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water by adding:
294.0 mg/l CaCl2 * 2 H2O
123.3 mg/l MgSO4 + 7 H2O
63.0 mg/l NaHCO3
5.5 mg/l KCl
- Continuous aeration with oil-free air

Test water ready for use:
Total hardness: 2.5 mmol/l
Acid capacity: 0.8 mmol/l
Ratio Ca/Mg ions: 4 : 1
Ratio Na/K ions: 10 : 1
pH: about 8.0

- Intervals of water quality measurement: temperature, pH and oxygen content after 0, 24, 48, 72 and 96 h

OTHER TEST CONDITIONS
- Photoperiod: : 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 1, 4, 24, 48, 72 and 96 h.
Endpoints investigated: mortality and symptoms of intoxication.

Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
46 - 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
21.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
21.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? yes
- LC50 (48h): 32 mg/L

Any other information on results incl. tables

Undissolved test substance was visible on the bottom increasing with increase in the concentration.

Applicant's summary and conclusion