Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Key value for biodegradation

Aerobic biodegradation in water: not persistent, 63% biodegradation at day 60

Based on the results of a reliable (RL1) enhanced (prolonged) ready biodegradability test (OECD 301 D) performed compliant with GLP, the submission substance must be regarded as not persistent (not P) in the environment (63% mineralisation based on O2 consumption within 60 days).

Additional information

In order to assess the biotic degradation, a ready biodegradability test was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test performed according to slightly modified OECD, EU and ISO Test Guidelines, and in compliance with the OECD principles of Good Laboratory Practice. River water was used as the inoculum.

Trinonyl benzene-1,2,4-tricarboxylate did not cause a reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum.

The test substance was biodegraded by 48% at day 28 in the Closed Bottle test.

In the prolonged Closed Bottle test, the test item was biodegraded by 63% at day 60 (enhanced biodegradability testing). This result demonstrates that the test substance is inherently biodegradable.

Moreover, a biodegradation percentage of >60 within a 60-day time period allows classification of trinonyl benzene-1,2,4-tricarboxylate as not persistent.

The test is valid as shown by an endogenous respiration of 1.1 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 76% of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met with oxygen concentrations remaining at >0.5 mg/L in all bottles over the test period. According to these results on biodegradation, the submission substance does not persist in the environment.