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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from U.S Environmental protection Agency report

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Investigation of selected Potential environmental contaminants: Nitroaromatics
Author:
Philip H. Howard, Joseph Santodonato, Jitendra Saxna, Judith Malling and Dorothy Greninger
Year:
1976
Bibliographic source:
U.S Environmental protection Agency, June 1976, page no 372
Reference Type:
secondary source
Title:
Acute Oral Toxicity of 3'-Nitroacetophenone
Author:
German Federal Ministry for the Environment
Year:
2016
Bibliographic source:
GSBL – Joint Substance Data Pool of the German Federal Government and the German Federal States, 2016

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as below
Principles of method if other than guideline:
Acute oral toxicity of 3'-Nitroacetophenone in rats
GLP compliance:
not specified
Test type:
other: No data
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
solid
Details on test material:
Name of test material (as cited in study report): 3 nitro acetophenone
Molecular formula : C8H7NO3
Molecular weight: 165.147 g/mol
Smiles notation : c1(cc(ccc1)[N+](=O)[O])C(C)=O
InChl : 1S/C8H7NO3/c16(10)73248(57)9(11)12/h25H,1H3
Substance type: Organic
Physical state: Solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3'-Nitroacetophenone
- Molecular formula (if other than submission substance): C8H7NO3
- Molecular weight (if other than submission substance): 165 g/mole
- Substance type: Organic

Test animals

Species:
rat
Strain:
other: Carworth Wistar
Sex:
male
Details on test animals and environmental conditions:
Weight at study initiation: 90 -120g
Fasting period before study: No fasting

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Details on oral exposure:
VEHICLE
Concentration in vehicle: 3250 mg/kg
Amount of vehicle (if gavage): 1 ml to 10 ml

CLASS METHOD (if applicable)
Rationale for the selection of the starting dose:
Doses were given in a logarithmic series differing by a factor of 2.

Doses:
3250 mg/kg bw
No. of animals per sex per dose:
5 male
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
Statistics:
The most probable LD50 value and its fiducial range are estimated by the method of Thompson, using the Tables of Weil. The figures in parentheses show limits of ± 1.96 standard deviations while the absence of parentheses indicates that no range is calculable because no dosage resulted in fractional mortality

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
3 250 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 % mortality observed.
Mortality:
50 % mortality was observed in treated rats at 3250 mg/kg bw after 14 days
Clinical signs:
No data available
Body weight:
No data available
Gross pathology:
No data available
Other findings:
No data available

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 was considered to be 3250 mg/kg bw when Carworth Wistar male rat were treated with 3'-Nitroacetophenone orally by gavage
Executive summary:

In a Acute oral toxicity study, 5 Carworth Wistar male rats were treated with 3'-Nitroacetophenone in the concentration of 3250 mg/kg bw orally by gavage and observed for 14 days. 50 % mortality was observed in treated rats at 3250 mg/kg bw after 14 days. Therefore, LD50 was considered to be 3250 mg/kg bw when Carworth Wistar male rat were treated with 3'-Nitroacetophenone orally by gavage.