Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The test item was homogenized in dilution water by ultrasonic and Ultra Turrax and then transferred into the test container

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling, Brachydanio rerio
- Strain: Hamilton Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation) about ten weeks:
- Length at study initiation: 2,4 - 3,6 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke. Melle; ad libitum
- Feeding frequency: twice a day


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
test group: 21,8 - 23,0 °C
control: 21,0 - 21,7
pH:
test group: 7,6 - 8,2
control: 8,0 - 8,3
Dissolved oxygen:
test group: 7,0 - 8,3 mg O2/L
control: 7,2 - 9,3 mg O2/L
Nominal and measured concentrations:
Nominal: 500 mg/L
Measured: 495 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass container (30 cm x 22 cm x 24 cm) calibtated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 h dark / 12 h light
- Light intensity: about 700 Lux

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Some fish showed decreased activity,irregular breathing, swim posture tail-heavy

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LD50 to fish is greater than 500 mh/L
Executive summary:

The test item was tested for toxicity to fish according to OECD guideline 203.Ten Brachydanio rerio (Zebrabärbling) were exposed to a concentration of nominal 500 mg/L. No lethality was observed after 96 hours.