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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January - February 2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study done to guideline, in compliance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
gas under pressure: refrigerated liquefied gas
Details on test material:
Chemical name (IUPAC) 3-(1-ethoxyethoxy)-3,7-dimethylocta-1,6-diene
CAS Number 40910-49-4
EC number 255-138-6
Molecular formula C14H26O2
Molecular weight 226.40 g/mol

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
Nominal: 10, 18, 32, 56 and 100 % of water soluble fraction (saturated solution)
Measured Initial: 2.2, 5.1, 9.2, 16 and 27 mg/l
Average exposure concentrations: 2.3, 4.5, 8.8, 15 and 24 mg/l.

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
12 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence interval between 11 and 13 mg/L
Details on results:
During the exposure period the measured concentrations remained relatively stable (78-102% of initial). However, given that not all the concentrations remained within 80-120% of the initial measured concentration, the EC50 was based on the average exposure concentrations that were calculated as 2.3, 4.5, 8.8, 15 and 24 mg/l.

Any other information on results incl. tables

The 48h-EC50 was 12 mg/l based on average exposure concentrations (95% confidence interval between 11 and 13 mg/l).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h-EC50 was 12 mg/l based on average exposure concentrations (95% confidence interval between 11 and 13 mg/l).