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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study, non-GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German Standard DIN 38412 L9
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5,6-trimethylcyclohex-2-en-1-one
EC Number:
243-473-0
EC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Cas Number:
20030-30-2
Molecular formula:
C9H14O
IUPAC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Details on test material:
- Name of test material (as cited in study report): 2,3,6-Trimethylcyclohexen-1-one

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
59.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
89.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
190.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass

Any other information on results incl. tables

Cell densities after x hours of exposure expressed as fluorometric values

Nominal test / Exposure time [h]
concentration /
(mg/l) / 0 / 24 / 48 / 72 / 96 /
Control / 11 / 137 / 297 / 1025 / 2947 /
3.91 / 12 / 140 / 301 / 992 / 2685 /
7.81 / 12 / 136 / 297 / 1009 / 2567 /
15.63 / 12 / 130 / 307 / 1060 / 2626 /
31.25 / 12 / 128 / 303 / 1106 / 2801 /
62.5 / 12 / 115 / 256 / 792 / 2285 /
125 / 12 / 78 / 116 / 149 / 258 /
250.0 / 12 / 48 / 51 / 60 / 70 /
500.0 / 12 / 19 / 15 / 21 / 22 /

fluorometric values in % of control
/ 72 h / 96 h
3.91 96.79 / 91.17
7.81 98.45 / 87.16
15.63 103.43 / 39.17
31.25 107.92 / 95.11
62.5 77.28 / 77.59
125 14.54 / 8.76
250.0 5.85 / 2.38
500.0 2.05 / 0.75


EC-values after 96 hours:
EC20: 58.2 mg/l
EC50: 87.6 mg/l
EC90: 123.9 mg/l

Applicant's summary and conclusion