Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study under GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide
IUPAC Name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source of inoculum/activated sludge: non-adapted sludge from domestic STP Hildesheim
- Pretreatment: washed twice

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20°C
Nominal and measured concentrations:
nominal: 100, 180, 320, 580, 1000 mg/l
Reference substance (positive control):
yes
Remarks:
Copper(II) sulfate pentahydrate

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50=105 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In the toxicity to microorganism study with a nominal loading of up to 1000 mg/L of the test item, no effects were observed.
Executive summary:

In the toxicity test to microorganism, the effects of the test item with a nominal loading of 100, 180, 320, 580 and1000 mg/L, were determined according to OECD 209 guideline.

A non-adapted sludge from domestic STP was used and no analytical monitoring of the test item was carried out.

The effect levels based on the nominal loading of test item are LC50>1000mg/l, NOEC=1000mg/l.

The validity criteria of the test guideline were fulfilled.

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