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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guidelins study under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide
IUPAC Name:
Reaction mass of Methyl N-octadecylterephthala​mate and N,​N'-​Dioctadecylterephtha​lamide
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
A concentration depending turbidity was observed. Despite the attempt to homogenize withe ultra-turrax the solutions remained inhomogenous.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling
- Source: Zoo Stumpe, Hildesheim
- Length at study initiation (mean): 3,0 cm
- Weight at study initiation (mean): 0,34 g
- Food type: Tetramin Haupfutter, Fa. Tetra

- Acclimation conditions: according to OECD guideline

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
132 mg/l CaCO3
Test temperature:
21-23°C
pH:
7,85-8,12
Dissolved oxygen:
90-99%
Nominal and measured concentrations:
nominal: 100, 180, 320, 580, 1000 mg/l
Details on test conditions:
- Test vessel: Aquarium, 26l
- Volume of medium per vessle: 10l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No mortality or non-letal effects were observed in any tested concentration (incl. control) at any time point.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In the acute toxicity to fish study with a nominal loading of up to 1000 mg/L of the test item, no effects on Danio rerio were
observed.
Executive summary:

In the acute toxicity test with Danio rerio, the effects of the test item with a nominal loading of 100, 180, 320, 580 and1000 mg/L, were determined according to OECD 203 guideline.

Ten fish were exposed to each concentration and the control.

No analytical monitoring of the test item was carried out.

The effect levels based on the nominal loading of test item are LC50>1000mg/l, NOEC=1000mg/l.

The validity criteria of the test guideline were fulfilled.

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