Methylammonium chloride

Endpoint summary

Description of key information

Short-term toxicity to fish:

The short-term toxicity of the Methylamine hydrochloride (CAS no.593 -51 -1) to Danio rerio (previous name: Brachydanio rerio) was predicted using QSAR toolbox version. 3.3 (2017), based on the effects observed in a static freshwater system during a 96 hr exposure.The lethal concentration (LC50) for the test compound Methylamine hydrochloride (CAS no.593 -51 -1) was estimated to be 494.55 mg/L. Thus, based on this value, test chemical Methylamine hydrochloride can be considered as non-toxic to fish and thus can be considered to be not classified as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Experimental study for inhibition of the mobility of daphnids was carried out with the Methylammonium chloride according to OECD Guideline 202(Report no716/16, Abitec 2016). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 100 mg/L. The test was performed under static conditions in a static fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.After the experiment, the inhibitory percentage was determined to be 0%,based on that the median effective concentration (EC50) for the test substance, Methylammonium chloride, in Daphnia magna was determined to be >100 mg/L on the basis of immobilisation effects.

Thus, based on this experimental value the Methylammonium chloride is not likely to be hazardous to aquatic invertebrates and can be considered as not classified as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance methanaminium chloride (CAS no. 593 -51 -1) according to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, Report no. 715/16, 2016) . The stock solution (300 mg/L) was prepared by dissolving white powder in OECD growth medium. Test solutions  of required concentrations  were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 60, 90, 132, 201 and 300 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance methanaminium chloride in Desmodesmus subspicatus was determined to be 971.6 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical methanaminium chloride can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

Additional information

Short-term toxicity to fish:

Two studies from prediction and experimental study using peer reviewed journal for short term fish toxicity endpoint of target chemical Methylamine hydrochloride (Cas no. 593 -51 -1) with relevant read across which is basic structure of the target chemical are summarized as followed:

The short-term toxicity of the Methylamine hydrochloride (CAS no.593 -51 -1) to Danio rerio (previous name: Brachydanio rerio) was predicted using QSAR toolbox version. 3.3 (2017), based on the effects observed in a static freshwater system during a 96 hr exposure.The lethal concentration (LC50) for the test compound Methylamine hydrochloride (CAS no.593 -51 -1) was estimated to be 494.55 mg/L. Thus, based on this value, test chemical Methylamine hydrochloride can be considered as non-toxic to fish and thus can be considered to be not classified as per the CLP classification criteria.

Above result for target chemical supported by experimental study of read across chemical Monomethylamine (Cas no.74-89-5) in which the toxic effect (TLm test) of aliphatic nitrogen compound Monomethylamine (Cas no.74-89-5) was observed. During experiment, ethanol used as a vehicle as chemical not easily dissolved in the water. 10 fish were used in the experiment. The test temperature maintained as 25 deg. C and analytical monitoring was done by the Gas chromatography.

At the end of the study the median lethal concentration (LC50) for chemical Monomethylamine (Cas no.74-89-5) was observed to be 1000 mg/l for 48 hrs exposure period on the basis of mortality effect.

All available median lethal concentration (LC50) values for fish toxicity in acute exposure is in the range of 494.55 -1000 mg/l which gives common conclusion as target chemical Methylamine hydrochloride (Cas no. 593 -51 -1) is considered to be non-toxic to fish and can be considered to be not classified for aquatic environment as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Experimental study for inhibition of the mobility of daphnids was carried out with the Methylammonium chloride according to OECD Guideline 202(Report no716/16, Abitec 2016). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 100 mg/L. The test was performed under static conditions in a static fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.After the experiment, the inhibitory percentage was determined to be 0%,based on that the median effective concentration (EC50) for the test substance, Methylammonium chloride, in Daphnia magna was determined to be >100 mg/L on the basis of immobilisation effects.

Thus, based on this experimental value the Methylammonium chloride is not likely to be hazardous to aquatic invertebrates and can be considered as not classified as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria

1 experimental key study alongwith the supporting datafor the target chemical methanaminium chloride(CAS no. 593-51-1)were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:

 

In an experimental key study (ABITEC study report, Sustainability Support Services (Europe) AB, Report no. 715/16, 2016), Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance methanaminium chloride (CAS no. 593 -51 -1) according to OECD Guideline 201. The stock solution (300 mg/L) was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 60, 90, 132, 201 and 300 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance methanaminium chloride in Desmodesmus subspicatus was determined to be 971.6 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical methanaminium chloride can be considered as non-toxic to aquatic organisms and thus can be considered to be not classified as per the CLP classification criteria.

 

In a supporting data (Fitzgerald,G.P., G.C. Gerloff, and F. Skoog, 1952) of short term toxicity to Microcystis aeruginosa for the test compound Methylamine hydrochloride (CAS no. 593 -51 -1) was carried out for 24 hrs in a static fresh water system at a temperature of 22ᵒC. Microcystis aeruginosa was used as a test organism. The algae was grown in 125 ml Erlenmeyer flasks with 75 ml of a modified Chu No. 10 solution under continuous florescent illumination and at a temperature of approx. 22ᵒC, respectively. Test chemical conc. used for the study was 20 mg/l(nominal concentrations). Test chemical was prepared in stock solutions and aliquots added to individual cultures to give the desired concentrations. Test was performed in a 125 ml Erlenmeyer flasks under continuous florescent illumination at a temperature of 22ᵒC. Initial cell of the organism used for the study was 1,000,000 to 2,000,000 cells/ml, respectively. Microscopic examination of the cells were carried out for determining the extent of their disintegration after 24 hr period. The failure of treated cultures to grow in subcultures was used for determining the minimum lethal dosages. Based on the effect on growth rate of the test organism Microcystis aeruginosa, the 24 hr EC100 value was determined to be 20 mg/l, respectively. Thus, based on the EC100 value, it can be concluded that the substance Methylamine hydrochloride can be considered as toxic to aquatic organisms. Since, the chemical Methylamine hydrochloride is readily biodegradable in nature, Methylamine hydrochloride can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

 

Thus, based on the overall reported results for target chemicalmethanaminium chloride(from ABITEC study report and peer reviewed journal), it can be concluded that the test substance methanaminium chloride can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.