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Short-term toxicity to aquatic invertebrates

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Description of key information

Exposure of the freshwater invertebrate Daphnia magna to an analogue test item gave a 48-Hour EC50 value of 2.6 mg/L (95% CI = 2.3 - 2.8 mg/L). The No Effect Concentration was 1.9 mg/L and the LOEC was considered to be 2.7 mg/L (OECD 202 and EU Method C.2).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
2.6 mg/L

Additional information

Introduction

A study was performed on an analogue substance to assess the acute toxicity of the test item to Daphnia magna and the read-across justification is presented in Section 13. The method followed was designed to becompatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp. Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

Methods

Due to the low aqueous solubility and pure nature of the test item, for the purposes of the definitive test, the test solutions were prepared from a slow stir saturated solution.

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to the test item over a range of nominal concentrations of 1.0, 1.8, 3.2, 5.6 and 10% v/v saturated solution for 48 hours at a temperature of approximately 21 °C under static test conditions. The numbers of immobilized Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.

Results

Chemical analysis of the 3.2, 5.6 and 10% v/v saturated solutions at 0 and 48 hours showed measured boron concentrations to range from 0.078 to 0.25 mg/L. TOC analysis of the 3.2, 5.6 and 10% v/v saturated solutions at 0 and 48 hours showed measured carbon concentrations to range from 1.4 to 3.7 mg/L (equivalent to test item concentrations of 1.9 to 4.9 mg/L based on a test item carbon content of 75.78%). It was considered appropriate to calculate the results based on the equivalent test item concentrations obtained from TOC analysis as these results give an estimate of the total dissolved test item concentrations present.

Exposure of the analogue test item to the freshwater invertebrate Daphnia magna gave a 48-Hour EC50 value of 2.6 mg/L (95% CI = 2.3 - 2.8 mg/L). The No Effect Concentration was 1.9 mg/L and the LOEC was considered to be 2.7 mg/L.